Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska; Sofie Wendel; Morten Nørholm; Antoine Danchin

doi:10.1101/041525

Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska, Sofie Wendel, Morten Nørholm, Antoine Danchin

Novo Nordisk Foundation Center for Biosustainability

Hôpital Pitié-Salpêtrière

Research output: Working paper/Preprint › Working paper › Research

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Abstract

How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome and over time, mutations increasingly originated from 8-oxo-guanosine, formed exclusively on the transcribed strand.

Original language	English
Publisher	bioRxiv
Number of pages	15
DOIs	https://doi.org/10.1101/041525
Publication status	Published - 2016

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abstract = "How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome and over time, mutations increasingly originated from 8-oxo-guanosine, formed exclusively on the transcribed strand.",

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Generation of mutation hotspots in ageing bacterial colonies

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