Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska, Sofie Wendel, Emil Christian Fischer, Morten H. H. Nørholm, Antoine Danchin

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How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.
Original languageEnglish
JournalScientific Reports
Issue number1
Number of pages7
Publication statusPublished - 2016


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