Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska, Sofie Wendel, Emil Christian Fischer, Morten H. H. Nørholm, Antoine Danchin

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Abstract

How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.
Original languageEnglish
JournalScientific Reports
Volume6
Issue number1
Number of pages7
ISSN2045-2322
DOIs
Publication statusPublished - 2016

Projects

BacTory: Biorefinery Training Platform: Bacterial Factories for Sustainable Chemical and Drug Production

Molin, S., Nørholm, M., Cavaleiro, M., Machado, H., Wendel, S., van der Helm, E., Bojanovic, K., Vazquez Albacete, D., D'Arrigo, I., Li, S., Yang, X., Redl, S. M. A., Pogrebnyakov, I., Lehning, C. E., Giubergia, S., Calero Valdayo, P. M. & Lohmann, R.

01/10/201231/01/2017

Project: Research

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