Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska; Sofie Wendel; Emil Christian Fischer; Morten H. H. Nørholm; Antoine Danchin

doi:10.1038/s41598-016-0005-4

Generation of mutation hotspots in ageing bacterial colonies

Agnieszka Sekowska, Sofie Wendel, Emil Christian Fischer, Morten H. H. Nørholm, Antoine Danchin

Novo Nordisk Foundation Center for Biosustainability

CHU, Paris

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

How do ageing bacterial colonies generate adaptive mutants? Over a period of two months, we isolated on ageing colonies outgrowing mutants able to use a new carbon source, and sequenced their genomes. This allowed us to uncover exquisite details on the molecular mechanism behind their adaptation: most mutations were located in just a few hotspots in the genome, and over time, mutations increasingly were consistent with the involvement of 8-oxo-guanosine, formed exclusively on the transcribed strand. This work provides strong support for retromutagenesis as a general process creating adaptive mutations during ageing.

Original language	English
Journal	Scientific Reports
Volume	6
Issue number	1
Number of pages	7
ISSN	2045-2322
DOIs	https://doi.org/10.1038/s41598-016-0005-4
Publication status	Published - 2016

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BacTory: Biorefinery Training Platform: Bacterial Factories for Sustainable Chemical and Drug Production
Molin, S., Nørholm, M., Cavaleiro, M., Machado, H., Wendel, S., van der Helm, E., Bojanovic, K., Vazquez Albacete, D., D'Arrigo, I., Li, S., Yang, X., Redl, S. M. A., Pogrebnyakov, I., Lehning, C. E., Giubergia, S., Calero Valdayo, P. M. & Lohmann, R.
01/10/2012 → 31/01/2017
Project: Research

Cite this

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Generation of mutation hotspots in ageing bacterial colonies

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BacTory: Biorefinery Training Platform: Bacterial Factories for Sustainable Chemical and Drug Production

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