Generalized phase contrast-enhanced diffractive coupling to light-driven microtools.

Mark Jayson Villangca, Andrew Rafael Bañas, Darwin Palima, Jesper Glückstad

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Abstract

We have previously demonstrated on-demand dynamic coupling to optically manipulated microtools coined as wave-guided optical waveguides using diffractive techniques on a “point and shoot” approach. These microtools are extended microstructures fabricated using two-photon photopolymerization and function as free-floating optically trapped waveguides. Dynamic coupling of focused light via these structures being moved in three-dimensional space is done holographically. However, calculating the necessary holograms is not straightforward when using counter-propagating trapping geometry. The generation of the coupling spots is done in real time following the position of each microtool with the aid of an object tracking routine. This approach allows continuous coupling of light through the microtools which can be useful in a variety of biophotonics applications. To complement the targeted-light delivery capability of the microtools, the applied spatial light modulator has been illuminated with a properly matched input beam cross section based on the generalized phase contrast method. Our results show a significant gain in the output at the tip of each microtool as measured from the fluorescence signal of the trapping medium. The ability to switch from on-demand to continuous addressing with efficient illumination leverages our microtools for potential applications in stimulation and near-field-based biophotonics on cellular scales
Original languageEnglish
Article number111308
JournalOptical Engineering
Volume54
Issue number11
Number of pages8
ISSN0091-3286
DOIs
Publication statusPublished - 2015

Bibliographical note

© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.OE.54.11.111308]

Keywords

  • Microfabrication
  • Optical Trapping
  • Object tracking
  • Generalized phase contrast
  • Holography

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