Abstract
In soil, bacteria can form colonies that are exposed to changing hydration conditions, exerting a stress to which the bacteria should adjust. Some of the phenotypes associated with water deprivation, such as the production extracellular polymeric substance (EPS) and the limitation of motility, have previously been observed. However, it is not known how these responses are regulated and temporally expressed. Here, we aimed to investigate the gene level responses by identifying the differentially expressed genes at -0.4 MPa water stress compared to non-stressed condition. We hypothesized that under water stress flagellar and EPS genes would be significantly differentially expressed, the former being down- and the latter being up-regulated.
The novel Pressurized Porous Surface Model (PPSM) was used to expose KT2440 colonies to -0.4 MPa water stress for 4, 24, and 72 hours. Agilent whole genome 1-color cDNA-microarray was used for gene expression profiling. The genes with log2-fold change >=1.5 and adjusted P-value
| Original language | English |
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| Title of host publication | ISME 13 |
| Volume | PS 29.021 |
| Publisher | International Society for Microbiology |
| Publication date | 2010 |
| Publication status | Published - 2010 |
| Event | 13th International Symposium on Microbial Ecology - Seattle, WA, United States Duration: 22 Aug 2010 → 27 Aug 2010 Conference number: 13 http://www.isme-microbes.org/isme13 |
Conference
| Conference | 13th International Symposium on Microbial Ecology |
|---|---|
| Number | 13 |
| Country/Territory | United States |
| City | Seattle, WA |
| Period | 22/08/2010 → 27/08/2010 |
| Internet address |