Gene expression dynamics of pseudomonas putida KT2440 biofilms under water deprivation

Gamze Gulez, Arnaud Dechesne, Christopher Workman, Barth F. Smets

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    In soil, bacteria can form colonies that are exposed to changing hydration conditions, exerting a stress to which the bacteria should adjust. Some of the phenotypes associated with water deprivation, such as the production extracellular polymeric substance (EPS) and the limitation of motility, have previously been observed. However, it is not known how these responses are regulated and temporally expressed. Here, we aimed to investigate the gene level responses by identifying the differentially expressed genes at -0.4 MPa water stress compared to non-stressed condition. We hypothesized that under water stress flagellar and EPS genes would be significantly differentially expressed, the former being down- and the latter being up-regulated. The novel Pressurized Porous Surface Model (PPSM) was used to expose KT2440 colonies to -0.4 MPa water stress for 4, 24, and 72 hours. Agilent whole genome 1-color cDNA-microarray was used for gene expression profiling. The genes with log2-fold change >=1.5 and adjusted P-value
    Original languageEnglish
    Title of host publicationISME 13
    VolumePS 29.021
    PublisherInternational Society for Microbiology
    Publication date2010
    Publication statusPublished - 2010
    Event13th International Symposium on Microbial Ecology - Seattle, WA, United States
    Duration: 22 Aug 201027 Aug 2010
    Conference number: 13


    Conference13th International Symposium on Microbial Ecology
    Country/TerritoryUnited States
    CitySeattle, WA
    Internet address


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