Gene expression dynamics of pseudomonas putida KT2440 biofilms under water deprivation

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Abstract

In soil, bacteria can form colonies that are exposed to changing hydration conditions, exerting a stress to which the bacteria should adjust. Some of the phenotypes associated with water deprivation, such as the production extracellular polymeric substance (EPS) and the limitation of motility, have previously been observed. However, it is not known how these responses are regulated and temporally expressed. Here, we aimed to investigate the gene level responses by identifying the differentially expressed genes at -0.4 MPa water stress compared to non-stressed condition. We hypothesized that under water stress flagellar and EPS genes would be significantly differentially expressed, the former being down- and the latter being up-regulated. The novel Pressurized Porous Surface Model (PPSM) was used to expose KT2440 colonies to -0.4 MPa water stress for 4, 24, and 72 hours. Agilent whole genome 1-color cDNA-microarray was used for gene expression profiling. The genes with log2-fold change >=1.5 and adjusted P-value
Original languageEnglish
Title of host publicationISME 13
VolumePS 29.021
PublisherInternational Society for Microbiology
Publication date2010
Publication statusPublished - 2010
Event13th International Symposium on Microbial Ecology - Seattle, WA, United States
Duration: 22 Aug 201027 Aug 2010
Conference number: 13
http://www.isme-microbes.org/isme13

Conference

Conference13th International Symposium on Microbial Ecology
Number13
CountryUnited States
CitySeattle, WA
Period22/08/201027/08/2010
Internet address

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