Gelatin from Saithe (Pollachius virens) Skin: Biochemical Characterization and Oxidative Stability in O/W Emulsions

Betül Yesiltas; Chloé Robert; Heidi Olander Petersen; Flemming Jessen; Fatemeh Ajalloueian; Mohammad Amin Mohammadifar; Charlotte Jacobsen; Jens J. Sloth; Greta Jakobsen; Federico Casanova

doi:10.3390/md20120739

Gelatin from Saithe (Pollachius virens) Skin: Biochemical Characterization and Oxidative Stability in O/W Emulsions

Betül Yesiltas^*, Chloé Robert, Heidi Olander Petersen, Flemming Jessen, Fatemeh Ajalloueian, Mohammad Amin Mohammadifar, Charlotte Jacobsen, Jens J. Sloth, Greta Jakobsen, Federico Casanova^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

This study performed the extraction of gelatin from saithe (Pollachius virens) skin and compared it to commercial marine gelatin. As a first stage, we investigated the physicochemical and biochemical properties of the gelatin. SDS-PAGE analysis revealed the presence of α-chains, β-chains, and other high-molecular-weight aggregates. DSC thermograms showed typical gelatin behavior, while the FTIR spectra were mainly situated in the amide band region (amide A, amide B, amide I, amide II, and amide III). In the second stage, we produced O/W emulsions and analyzed their physical and oxidative stability over 9 days. Oil droplets stabilized with the gelatins obtained from saithe fish skin had a size of ~500 nm and a ζ-potential ~+25 mV, which is comparable to oil droplets stabilized with commercial gelatin products. Moreover, the oxidative stability of the emulsions stabilized with gelatin from saithe fish skin showed promising results in terms of preventing the formation of some volatile compounds towards the end of the storage period compared to when using the commercial gelatins. This study indicates the potential application of fish skin gelatin in the fields of food and cosmetics, as well as suggesting that further investigations of their techno-functional properties.

Original language	English
Article number	739
Journal	Marine Drugs
Volume	20
Issue number	12
Number of pages	17
ISSN	1660-3397
DOIs	https://doi.org/10.3390/md20120739
Publication status	Published - 2022

Bibliographical note

This research was funded by the Green Development and Demonstration Program (GUDP) and Ministry of Environment and Food of Denmark (J. Nr. 34009‐17‐1299). The authors acknowledge the founding ERASMUS Internship for Chloé Robert.

Keywords

Fish skin gelatin
Physical and oxidative stability
Oil‐in‐water emulsions

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "Gelatin from Saithe (Pollachius virens) Skin: Biochemical Characterization and Oxidative Stability in O/W Emulsions",

abstract = " This study performed the extraction of gelatin from saithe (Pollachius virens) skin and compared it to commercial marine gelatin. As a first stage, we investigated the physicochemical and biochemical properties of the gelatin. SDS-PAGE analysis revealed the presence of α-chains, β-chains, and other high-molecular-weight aggregates. DSC thermograms showed typical gelatin behavior, while the FTIR spectra were mainly situated in the amide band region (amide A, amide B, amide I, amide II, and amide III). In the second stage, we produced O/W emulsions and analyzed their physical and oxidative stability over 9 days. Oil droplets stabilized with the gelatins obtained from saithe fish skin had a size of ~500 nm and a ζ-potential ~+25 mV, which is comparable to oil droplets stabilized with commercial gelatin products. Moreover, the oxidative stability of the emulsions stabilized with gelatin from saithe fish skin showed promising results in terms of preventing the formation of some volatile compounds towards the end of the storage period compared to when using the commercial gelatins. This study indicates the potential application of fish skin gelatin in the fields of food and cosmetics, as well as suggesting that further investigations of their techno-functional properties.",

keywords = "Fish skin gelatin, Physical and oxidative stability, Oil‐in‐water emulsions",

author = "Bet{\"u}l Yesiltas and Chlo{\'e} Robert and Petersen, {Heidi Olander} and Flemming Jessen and Fatemeh Ajalloueian and Mohammadifar, {Mohammad Amin} and Charlotte Jacobsen and Sloth, {Jens J.} and Greta Jakobsen and Federico Casanova",

note = "This research was funded by the Green Development and Demonstration Program (GUDP) and Ministry of Environment and Food of Denmark (J. Nr. 34009‐17‐1299). The authors acknowledge the founding ERASMUS Internship for Chlo{\'e} Robert.",

year = "2022",

doi = "10.3390/md20120739",

language = "English",

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journal = "Marine Drugs",

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T1 - Gelatin from Saithe (Pollachius virens) Skin

T2 - Biochemical Characterization and Oxidative Stability in O/W Emulsions

AU - Yesiltas, Betül

AU - Robert, Chloé

AU - Petersen, Heidi Olander

AU - Jessen, Flemming

AU - Ajalloueian, Fatemeh

AU - Mohammadifar, Mohammad Amin

AU - Jacobsen, Charlotte

AU - Sloth, Jens J.

AU - Jakobsen, Greta

AU - Casanova, Federico

N1 - This research was funded by the Green Development and Demonstration Program (GUDP) and Ministry of Environment and Food of Denmark (J. Nr. 34009‐17‐1299). The authors acknowledge the founding ERASMUS Internship for Chloé Robert.

PY - 2022

Y1 - 2022

N2 - This study performed the extraction of gelatin from saithe (Pollachius virens) skin and compared it to commercial marine gelatin. As a first stage, we investigated the physicochemical and biochemical properties of the gelatin. SDS-PAGE analysis revealed the presence of α-chains, β-chains, and other high-molecular-weight aggregates. DSC thermograms showed typical gelatin behavior, while the FTIR spectra were mainly situated in the amide band region (amide A, amide B, amide I, amide II, and amide III). In the second stage, we produced O/W emulsions and analyzed their physical and oxidative stability over 9 days. Oil droplets stabilized with the gelatins obtained from saithe fish skin had a size of ~500 nm and a ζ-potential ~+25 mV, which is comparable to oil droplets stabilized with commercial gelatin products. Moreover, the oxidative stability of the emulsions stabilized with gelatin from saithe fish skin showed promising results in terms of preventing the formation of some volatile compounds towards the end of the storage period compared to when using the commercial gelatins. This study indicates the potential application of fish skin gelatin in the fields of food and cosmetics, as well as suggesting that further investigations of their techno-functional properties.

AB - This study performed the extraction of gelatin from saithe (Pollachius virens) skin and compared it to commercial marine gelatin. As a first stage, we investigated the physicochemical and biochemical properties of the gelatin. SDS-PAGE analysis revealed the presence of α-chains, β-chains, and other high-molecular-weight aggregates. DSC thermograms showed typical gelatin behavior, while the FTIR spectra were mainly situated in the amide band region (amide A, amide B, amide I, amide II, and amide III). In the second stage, we produced O/W emulsions and analyzed their physical and oxidative stability over 9 days. Oil droplets stabilized with the gelatins obtained from saithe fish skin had a size of ~500 nm and a ζ-potential ~+25 mV, which is comparable to oil droplets stabilized with commercial gelatin products. Moreover, the oxidative stability of the emulsions stabilized with gelatin from saithe fish skin showed promising results in terms of preventing the formation of some volatile compounds towards the end of the storage period compared to when using the commercial gelatins. This study indicates the potential application of fish skin gelatin in the fields of food and cosmetics, as well as suggesting that further investigations of their techno-functional properties.

KW - Fish skin gelatin

KW - Physical and oxidative stability

KW - Oil‐in‐water emulsions

U2 - 10.3390/md20120739

DO - 10.3390/md20120739

M3 - Journal article

C2 - 36547886

SN - 1660-3397

VL - 20

JO - Marine Drugs

JF - Marine Drugs

IS - 12

M1 - 739

ER -

Gelatin from Saithe (Pollachius virens) Skin: Biochemical Characterization and Oxidative Stability in O/W Emulsions

Abstract

Bibliographical note

Keywords

UN SDGs

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