Gamma camera imaging for studying intestinal absorption and whole-body distribution of selenomethionine

Jan L. Madsen, Katarina Sjögreen-Gleisner, Dennis Ringkjøbing Elema, Lasse R. Søndergaard, Palle Rasmussen, Stefan Fuglsang, Michael Ljungberg, Morten Damgaard

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Abstract

Se metabolism in humans is not well characterised. Currently, the estimates of Se absorption, whole-body retention and excretion are being obtained from balance and tracer studies. In the present study, we used gamma camera imaging to evaluate the whole-body retention and distribution of radiolabelled selenomethionine (SeMet), the predominant form of Se present in foods. A total of eight healthy young men participated in the study. After consumption of a meal containing 4MBq [75Se]L-SeMet ([75Se]SeMet), whole-body gamma camera scanning was performed for 45 min every hour over a 6 h period, every second hour for the next 18 h and once on each of the subsequent 6 d. Blood, urine and faecal samples were collected to determine the plasma content of [75Se]SeMet as well as its excretion in urine and faeces. Imaging showed that 87•9 (SD 3•3)% of the administered activity of [75Se]SeMet was retained within the body after 7 d. In contrast, the measured excretion in urine and faeces for the 7 d period was 8•2 (SD 1•1)% of the activity. Time–activity curves were generated for the whole body, stomach, liver, abdomen (other than the stomach and the liver), brain and femoral muscles. Gamma camera imaging allows for the assessment of the postprandial absorption of SeMet. This technique may also permit concurrent studies of organ turnover of SeMet.
Original languageEnglish
JournalBritish Journal of Nutrition
Volume111
Issue number3
Pages (from-to)547-553
ISSN0007-1145
DOIs
Publication statusPublished - 2014

Bibliographical note

© The Authors 2013

Keywords

  • Selenomethionine
  • Absorption
  • Whole-body distribution
  • Gamma camera imaging

Cite this

Madsen, J. L., Sjögreen-Gleisner, K., Elema, D. R., Søndergaard, L. R., Rasmussen, P., Fuglsang, S., ... Damgaard, M. (2014). Gamma camera imaging for studying intestinal absorption and whole-body distribution of selenomethionine. British Journal of Nutrition, 111(3), 547-553. https://doi.org/10.1017/S0007114513002559
Madsen, Jan L. ; Sjögreen-Gleisner, Katarina ; Elema, Dennis Ringkjøbing ; Søndergaard, Lasse R. ; Rasmussen, Palle ; Fuglsang, Stefan ; Ljungberg, Michael ; Damgaard, Morten. / Gamma camera imaging for studying intestinal absorption and whole-body distribution of selenomethionine. In: British Journal of Nutrition. 2014 ; Vol. 111, No. 3. pp. 547-553.
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Madsen, JL, Sjögreen-Gleisner, K, Elema, DR, Søndergaard, LR, Rasmussen, P, Fuglsang, S, Ljungberg, M & Damgaard, M 2014, 'Gamma camera imaging for studying intestinal absorption and whole-body distribution of selenomethionine', British Journal of Nutrition, vol. 111, no. 3, pp. 547-553. https://doi.org/10.1017/S0007114513002559

Gamma camera imaging for studying intestinal absorption and whole-body distribution of selenomethionine. / Madsen, Jan L.; Sjögreen-Gleisner, Katarina; Elema, Dennis Ringkjøbing; Søndergaard, Lasse R.; Rasmussen, Palle; Fuglsang, Stefan; Ljungberg, Michael; Damgaard, Morten.

In: British Journal of Nutrition, Vol. 111, No. 3, 2014, p. 547-553.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Gamma camera imaging for studying intestinal absorption and whole-body distribution of selenomethionine

AU - Madsen, Jan L.

AU - Sjögreen-Gleisner, Katarina

AU - Elema, Dennis Ringkjøbing

AU - Søndergaard, Lasse R.

AU - Rasmussen, Palle

AU - Fuglsang, Stefan

AU - Ljungberg, Michael

AU - Damgaard, Morten

N1 - © The Authors 2013

PY - 2014

Y1 - 2014

N2 - Se metabolism in humans is not well characterised. Currently, the estimates of Se absorption, whole-body retention and excretion are being obtained from balance and tracer studies. In the present study, we used gamma camera imaging to evaluate the whole-body retention and distribution of radiolabelled selenomethionine (SeMet), the predominant form of Se present in foods. A total of eight healthy young men participated in the study. After consumption of a meal containing 4MBq [75Se]L-SeMet ([75Se]SeMet), whole-body gamma camera scanning was performed for 45 min every hour over a 6 h period, every second hour for the next 18 h and once on each of the subsequent 6 d. Blood, urine and faecal samples were collected to determine the plasma content of [75Se]SeMet as well as its excretion in urine and faeces. Imaging showed that 87•9 (SD 3•3)% of the administered activity of [75Se]SeMet was retained within the body after 7 d. In contrast, the measured excretion in urine and faeces for the 7 d period was 8•2 (SD 1•1)% of the activity. Time–activity curves were generated for the whole body, stomach, liver, abdomen (other than the stomach and the liver), brain and femoral muscles. Gamma camera imaging allows for the assessment of the postprandial absorption of SeMet. This technique may also permit concurrent studies of organ turnover of SeMet.

AB - Se metabolism in humans is not well characterised. Currently, the estimates of Se absorption, whole-body retention and excretion are being obtained from balance and tracer studies. In the present study, we used gamma camera imaging to evaluate the whole-body retention and distribution of radiolabelled selenomethionine (SeMet), the predominant form of Se present in foods. A total of eight healthy young men participated in the study. After consumption of a meal containing 4MBq [75Se]L-SeMet ([75Se]SeMet), whole-body gamma camera scanning was performed for 45 min every hour over a 6 h period, every second hour for the next 18 h and once on each of the subsequent 6 d. Blood, urine and faecal samples were collected to determine the plasma content of [75Se]SeMet as well as its excretion in urine and faeces. Imaging showed that 87•9 (SD 3•3)% of the administered activity of [75Se]SeMet was retained within the body after 7 d. In contrast, the measured excretion in urine and faeces for the 7 d period was 8•2 (SD 1•1)% of the activity. Time–activity curves were generated for the whole body, stomach, liver, abdomen (other than the stomach and the liver), brain and femoral muscles. Gamma camera imaging allows for the assessment of the postprandial absorption of SeMet. This technique may also permit concurrent studies of organ turnover of SeMet.

KW - Selenomethionine

KW - Absorption

KW - Whole-body distribution

KW - Gamma camera imaging

U2 - 10.1017/S0007114513002559

DO - 10.1017/S0007114513002559

M3 - Journal article

VL - 111

SP - 547

EP - 553

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 3

ER -

Madsen JL, Sjögreen-Gleisner K, Elema DR, Søndergaard LR, Rasmussen P, Fuglsang S et al. Gamma camera imaging for studying intestinal absorption and whole-body distribution of selenomethionine. British Journal of Nutrition. 2014;111(3):547-553. https://doi.org/10.1017/S0007114513002559