Göttingen minipig model of diet-induced atherosclerosis: influence of mild streptozotocin-induced diabetes on lesion severity and markers of inflammation evaluated in obese, obese and diabetic, and lean control animals

Trine Pagh Ludvigsen, Rikke Kaae Kirk, Berit Østergaard Christoffersen, Henrik Pedersen, Torben Martinussen, Jonas Kildegaard, Peter M. H. Heegaard, Jens Lykkesfeldt, Lisbeth Høier Olsen

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    Abstract

    From a pharmacological perspective, readily-available, well-characterized animal models of cardiovascular disease, including relevant in vivo markers of atherosclerosis are important for evaluation of novel drug candidates. Furthermore, considering the impact of diabetes mellitus on atherosclerosis in human patients, inclusion of this disease aspect in the characterization of a such model, is highly relevant. The objective of this study was to evaluate the effect of mild streptozotocin-induced diabetes on ex- and in vivo end-points in a diet-induced atherosclerotic minipig model. Castrated male Göttingen minipigs were fed standard chow (CD), atherogenic diet alone (HFD) or with superimposed mild streptozotocin-induced diabetes (HFD-D). Circulating markers of inflammation (C-reactive protein (CRP), oxidized low-density lipoprotein (oxLDL), plasminogen activator inhibitor-1, lipid and glucose metabolism were evaluated together with coronary and aortic atherosclerosis after 22 or 43 diet-weeks. Group differences were evaluated by analysis of variance for parametric data and Kruskal-Wallis test for non-parametric data. For qualitative assessments, Fisher's exact test was applied. For all analyses, p 
    Original languageEnglish
    Article number312
    JournalJournal of Translational Medicine
    Volume13
    Issue number1
    Number of pages12
    ISSN1479-5876
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Atherosclerosis
    • Animal model
    • Pig
    • Cardiovascular disease
    • Biomarkers
    • Inflammation
    • Obesity
    • Diabetes mellitus

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