Further biochemical studies on aminopyrrolnitrin oxygenase (PrnD)

Manish Kumar Tiwari, Jung-Kul Lee, Hee-Jung Moon, Huimin Zhao

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Active site modeling of dimerization interface in combination with site-directed mutagenesis indicates that the electron in the PrnD Rieske oxygenase can be transferred by either of two pathways, one involving Asp183' and the other involving Asn180'. In addition, the overexpression of the isc operon involved in the assembly of iron-sulfur clusters increased the catalytic activity of PrnD in Escherichia coli by a factor of at least 4. (C) 2011 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalBioorganic & Medicinal Chemistry Letters
Volume21
Issue number10
Pages (from-to)2873-2876
Number of pages4
ISSN0960-894X
DOIs
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • Amines
  • Binding Sites
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli
  • Models, Molecular
  • Mutagenesis, Site-Directed
  • Oxygenases
  • Pyrrolnitrin
  • EC 1.13.- Oxygenases
  • N0P24B6EDQ Pyrrolnitrin
  • aminopyrrolnitrin oxygenase
  • asparagine
  • aspartic acid
  • iron
  • oxygenase
  • protein PrnD
  • sulfur
  • unclassified drug
  • article
  • crystal structure
  • electron
  • electron transport
  • enzyme active site
  • enzyme activity
  • enzyme structure
  • expression vector
  • molecular model
  • nonhuman
  • operon
  • protein domain
  • site directed mutagenesis
  • Active site modeling
  • Aminopyrrolnitrin oxygenase (PrnD)
  • Electron transfer
  • Isc operon
  • Overexpression
  • CHEMISTRY,
  • IRON-SULFUR CLUSTERS
  • ESCHERICHIA-COLI
  • AZOTOBACTER-VINELANDII
  • GENE-CLUSTER
  • N-OXYGENASE
  • IN-VIVO
  • IDENTIFICATION
  • PYRROLNITRIN
  • DIOXYGENASE
  • OXIDATION
  • biochemical pathway
  • Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli species
  • Mycobacteria Actinomycetes and Related Organisms Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Mycobacteriaceae [08881] Mycobacterium tuberculosis species
  • Escherichia coli isc gene [Enterobacteriaceae]
  • aminopyrrolnitrin oxygenase PrnD
  • iron-sulfur cluster
  • Rieske oxygenase
  • 03502, Genetics - General
  • 04500, Mathematical biology and statistical methods
  • 10515, Biophysics - Biocybernetics
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 31000, Physiology and biochemistry of bacteria
  • 31500, Genetics of bacteria and viruses
  • Biochemistry and Molecular Biophysics
  • Computational Biology
  • active site modeling mathematical and computer techniques
  • site-directed mutagenesis laboratory techniques, genetic techniques
  • Enzymology
  • Models and Simulations
  • Molecular Genetics

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