Fungal metabolite screening: database of 474 mycotoxins and fungal metabolites for dereplication by standardised liquid chromatography-UV-mass spectrometry methodology

Kristian Fog Nielsen, Jørn Smedsgaard

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A standardised LC-UV-MS micro-scale method for screening of fungal metabolites and mycotoxins in culture extracts is presented. The paper includes data for detection and dereplication of >400 fungal metabolites to facilitate detection and identification when standards are not available. The data also shows the types of components that can be analysed by positive electrospray (ESI+) mass spectrometry (MS) along with common fragments and adducts of these, as well as giving suggestions on whether UV or ESI+ -MS methods should be used. Examples of dereplication of penitrems and macro-cyclic trichothecenes, and detection of several novel compounds are shown. This was done by UV spectroscopy combined with accurate mass determination of adduct and fragment ions obtained by high-resolution orthogonal time-of-flight MS.
    Original languageEnglish
    JournalJournal of Chromatography A
    Volume1002
    Issue number1-2
    Pages (from-to)111-136
    ISSN0021-9673
    Publication statusPublished - 2003

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