Fungal hyphae stimulate bacterial degradation of 2,6-dichlorobenzamide (BAM)

Berith Elkær Knudsen, Lea Ellegaard-Jensen, Christian Nyrop Albers, Soren Rosendahl, Jens Aamand

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Abstract

Introduction of specific degrading microorganisms into polluted soil or aquifers is a promising remediation technology provided that the organisms survive and spread in the environment. We suggest that consortia, rather than single strains, may be better suited to overcome these challenges. Here we introduced a fungal bacterial consortium consisting of Mortierella sp. LEJ702 and the 2,6-dichlorobenzamide (BAM)-degrading Aminobacter sp. MSH1 into small sand columns. A more rapid mineralisation of BAM was obtained by the consortium compared to MSH1 alone especially at lower moisture contents. Results from quantitative real-time polymerase chain reaction (qPCR) demonstrated better spreading of Aminobacter when Mortierella was present suggesting that fungal hyphae may stimulate bacterial dispersal. Extraction and analysis of BAM indicated that translocation of the compound was also affected by the fungal hyphae in the sand. This suggests that fungal bacterial consortia are promising for successful bioremediation of pesticide contamination. (C) 2013 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalEnvironmental Pollution
Volume181
Pages (from-to)122-127
Number of pages6
ISSN0269-7491
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • 2,6-dichlorobenzamide (BAM)
  • Consortium
  • Bacterial dispersal
  • Pesticide biodegradation
  • Fungal–bacterial interactions
  • ENVIRONMENTAL
  • DEGRADING BACTERIA
  • SOIL
  • BIOAUGMENTATION
  • MINERALIZATION
  • BIOREMEDIATION
  • REMEDIATION
  • TRANSPORT
  • SELECTION
  • SURVIVAL
  • FIELD
  • Fungal-bacterial interactions
  • aquifer
  • bioremediation
  • fungal bacterial consortium
  • mineralisation
  • moisture content
  • polluted soil
  • Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Phycomycetes [15900] Mortierella genus
  • Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Pseudomonadaceae [06508] Aminobacter genus
  • Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] bacteria common
  • Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi [15000] fungi common
  • 2,6-dichlorobenzamide pesticide, pollutant degradation
  • 31000, Physiology and biochemistry of bacteria
  • 37015, Public health - Air, water and soil pollution
  • 39008, Food microbiology - General and miscellaneous
  • 54512, Phytopathology - Nonparasitic diseases
  • 54600, Pest control: general, pesticides and herbicides
  • hyphe
  • quantitative real-time polymerase chain reaction qPCR laboratory techniques, genetic techniques
  • Bioprocess Engineering
  • Pesticides
  • Pollution Assessment Control and Management
  • Bacteria
  • Benzamides
  • Biodegradation, Environmental
  • Environmental Pollutants
  • Environmental Pollution
  • Groundwater
  • Hyphae
  • Soil Microbiology
  • Water Microbiology
  • 2008-58-4 2,6-dichlorobenzamide
  • Aquifers
  • Biodegradation
  • Bioremediation
  • Fungi
  • Hydrogeology
  • Microbiology
  • Polymerase chain reaction
  • Soil pollution
  • Bacterial degradation
  • Pesticide contaminations
  • Quantitative real-time polymerase chain reaction
  • Remediation technologies

Cite this

Knudsen, B. E., Ellegaard-Jensen, L., Albers, C. N., Rosendahl, S., & Aamand, J. (2013). Fungal hyphae stimulate bacterial degradation of 2,6-dichlorobenzamide (BAM). Environmental Pollution, 181, 122-127. https://doi.org/10.1016/j.envpol.2013.06.013
Knudsen, Berith Elkær ; Ellegaard-Jensen, Lea ; Albers, Christian Nyrop ; Rosendahl, Soren ; Aamand, Jens. / Fungal hyphae stimulate bacterial degradation of 2,6-dichlorobenzamide (BAM). In: Environmental Pollution. 2013 ; Vol. 181. pp. 122-127.
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Knudsen, BE, Ellegaard-Jensen, L, Albers, CN, Rosendahl, S & Aamand, J 2013, 'Fungal hyphae stimulate bacterial degradation of 2,6-dichlorobenzamide (BAM)', Environmental Pollution, vol. 181, pp. 122-127. https://doi.org/10.1016/j.envpol.2013.06.013

Fungal hyphae stimulate bacterial degradation of 2,6-dichlorobenzamide (BAM). / Knudsen, Berith Elkær; Ellegaard-Jensen, Lea; Albers, Christian Nyrop; Rosendahl, Soren; Aamand, Jens.

In: Environmental Pollution, Vol. 181, 2013, p. 122-127.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Fungal hyphae stimulate bacterial degradation of 2,6-dichlorobenzamide (BAM)

AU - Knudsen, Berith Elkær

AU - Ellegaard-Jensen, Lea

AU - Albers, Christian Nyrop

AU - Rosendahl, Soren

AU - Aamand, Jens

PY - 2013

Y1 - 2013

N2 - Introduction of specific degrading microorganisms into polluted soil or aquifers is a promising remediation technology provided that the organisms survive and spread in the environment. We suggest that consortia, rather than single strains, may be better suited to overcome these challenges. Here we introduced a fungal bacterial consortium consisting of Mortierella sp. LEJ702 and the 2,6-dichlorobenzamide (BAM)-degrading Aminobacter sp. MSH1 into small sand columns. A more rapid mineralisation of BAM was obtained by the consortium compared to MSH1 alone especially at lower moisture contents. Results from quantitative real-time polymerase chain reaction (qPCR) demonstrated better spreading of Aminobacter when Mortierella was present suggesting that fungal hyphae may stimulate bacterial dispersal. Extraction and analysis of BAM indicated that translocation of the compound was also affected by the fungal hyphae in the sand. This suggests that fungal bacterial consortia are promising for successful bioremediation of pesticide contamination. (C) 2013 Elsevier Ltd. All rights reserved.

AB - Introduction of specific degrading microorganisms into polluted soil or aquifers is a promising remediation technology provided that the organisms survive and spread in the environment. We suggest that consortia, rather than single strains, may be better suited to overcome these challenges. Here we introduced a fungal bacterial consortium consisting of Mortierella sp. LEJ702 and the 2,6-dichlorobenzamide (BAM)-degrading Aminobacter sp. MSH1 into small sand columns. A more rapid mineralisation of BAM was obtained by the consortium compared to MSH1 alone especially at lower moisture contents. Results from quantitative real-time polymerase chain reaction (qPCR) demonstrated better spreading of Aminobacter when Mortierella was present suggesting that fungal hyphae may stimulate bacterial dispersal. Extraction and analysis of BAM indicated that translocation of the compound was also affected by the fungal hyphae in the sand. This suggests that fungal bacterial consortia are promising for successful bioremediation of pesticide contamination. (C) 2013 Elsevier Ltd. All rights reserved.

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KW - Pesticide biodegradation

KW - Fungal–bacterial interactions

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KW - DEGRADING BACTERIA

KW - SOIL

KW - BIOAUGMENTATION

KW - MINERALIZATION

KW - BIOREMEDIATION

KW - REMEDIATION

KW - TRANSPORT

KW - SELECTION

KW - SURVIVAL

KW - FIELD

KW - Fungal-bacterial interactions

KW - aquifer

KW - bioremediation

KW - fungal bacterial consortium

KW - mineralisation

KW - moisture content

KW - polluted soil

KW - Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Phycomycetes [15900] Mortierella genus

KW - Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Pseudomonadaceae [06508] Aminobacter genus

KW - Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteria [05000] bacteria common

KW - Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi [15000] fungi common

KW - 2,6-dichlorobenzamide pesticide, pollutant degradation

KW - 31000, Physiology and biochemistry of bacteria

KW - 37015, Public health - Air, water and soil pollution

KW - 39008, Food microbiology - General and miscellaneous

KW - 54512, Phytopathology - Nonparasitic diseases

KW - 54600, Pest control: general, pesticides and herbicides

KW - hyphe

KW - quantitative real-time polymerase chain reaction qPCR laboratory techniques, genetic techniques

KW - Bioprocess Engineering

KW - Pesticides

KW - Pollution Assessment Control and Management

KW - Bacteria

KW - Benzamides

KW - Biodegradation, Environmental

KW - Environmental Pollutants

KW - Environmental Pollution

KW - Groundwater

KW - Hyphae

KW - Soil Microbiology

KW - Water Microbiology

KW - 2008-58-4 2,6-dichlorobenzamide

KW - Aquifers

KW - Biodegradation

KW - Bioremediation

KW - Fungi

KW - Hydrogeology

KW - Microbiology

KW - Polymerase chain reaction

KW - Soil pollution

KW - Bacterial degradation

KW - Pesticide contaminations

KW - Quantitative real-time polymerase chain reaction

KW - Remediation technologies

U2 - 10.1016/j.envpol.2013.06.013

DO - 10.1016/j.envpol.2013.06.013

M3 - Journal article

VL - 181

SP - 122

EP - 127

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

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