Fungal fucoidanase production by solid-state fermentation in a rotating drum bioreactor using algal biomass as substrate

Rosa M. Rodríguez-Jasso, Solange I. Mussatto, Leonardo Sepúlveda, Ana Torrado-Agrasar, Lorenzo Pastrana , Cristobal N. Aguilar, Jose A. Teixeira

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Fucoidanase enzymes able to degrade fucoidan were produced by solid-state fermentation (SSF). The fermentation assays were initially carried out in a laboratory-scale rotating drum bioreactor, and two fungal strains (Aspergillus niger PSH and Mucor sp. 3P) and three algal substrates (untreated, autohydrolyzed, and microwave processed seaweed Fucus vesiculosus) were evaluated. Additionally, fermentations were carried out under rotational (10 rpm) and static conditions in order to determine the effect of the agitation on the enzyme production. Agitated experiments showed advantages in the induction of the enzyme when compared to the static ones. The conditions that promoted the maximum fucoidanase activity (3.82 U L−1) consisted in using Mucor sp. 3P as fungal strain, autohydrolyzed alga as substrate, and the rotational system. Such conditions were subsequently used in a 10 times larger scale rotating drum bioreactor. In this step, the effect of controlling the substrate moisture during the enzyme production by SSF was investigated. Moreover, assays combining the algal substrate with an inert support (synthetic fiber) were also carried out. Fermentation of the autohydrolyzed alga with the moisture content maintained at 80% during the fermentation with Mucor sp. 3P gave the highest enzyme activity (9.62 U L−1).
Original languageEnglish
JournalFood and Bioproducts Processing
Volume91
Issue number4
Pages (from-to)587–594
ISSN0960-3085
DOIs
Publication statusPublished - 2013
Externally publishedYes

Cite this

Rodríguez-Jasso, R. M., Mussatto, S. I., Sepúlveda, L., Torrado-Agrasar, A., Pastrana , L., Aguilar, C. N., & Teixeira, J. A. (2013). Fungal fucoidanase production by solid-state fermentation in a rotating drum bioreactor using algal biomass as substrate. Food and Bioproducts Processing, 91(4), 587–594. https://doi.org/10.1016/j.fbp.2013.02.004
Rodríguez-Jasso, Rosa M. ; Mussatto, Solange I. ; Sepúlveda, Leonardo ; Torrado-Agrasar, Ana ; Pastrana , Lorenzo ; Aguilar, Cristobal N. ; Teixeira, Jose A. . / Fungal fucoidanase production by solid-state fermentation in a rotating drum bioreactor using algal biomass as substrate. In: Food and Bioproducts Processing. 2013 ; Vol. 91, No. 4. pp. 587–594.
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abstract = "Fucoidanase enzymes able to degrade fucoidan were produced by solid-state fermentation (SSF). The fermentation assays were initially carried out in a laboratory-scale rotating drum bioreactor, and two fungal strains (Aspergillus niger PSH and Mucor sp. 3P) and three algal substrates (untreated, autohydrolyzed, and microwave processed seaweed Fucus vesiculosus) were evaluated. Additionally, fermentations were carried out under rotational (10 rpm) and static conditions in order to determine the effect of the agitation on the enzyme production. Agitated experiments showed advantages in the induction of the enzyme when compared to the static ones. The conditions that promoted the maximum fucoidanase activity (3.82 U L−1) consisted in using Mucor sp. 3P as fungal strain, autohydrolyzed alga as substrate, and the rotational system. Such conditions were subsequently used in a 10 times larger scale rotating drum bioreactor. In this step, the effect of controlling the substrate moisture during the enzyme production by SSF was investigated. Moreover, assays combining the algal substrate with an inert support (synthetic fiber) were also carried out. Fermentation of the autohydrolyzed alga with the moisture content maintained at 80{\%} during the fermentation with Mucor sp. 3P gave the highest enzyme activity (9.62 U L−1).",
author = "Rodr{\'i}guez-Jasso, {Rosa M.} and Mussatto, {Solange I.} and Leonardo Sep{\'u}lveda and Ana Torrado-Agrasar and Lorenzo Pastrana and Aguilar, {Cristobal N.} and Teixeira, {Jose A.}",
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Rodríguez-Jasso, RM, Mussatto, SI, Sepúlveda, L, Torrado-Agrasar, A, Pastrana , L, Aguilar, CN & Teixeira, JA 2013, 'Fungal fucoidanase production by solid-state fermentation in a rotating drum bioreactor using algal biomass as substrate', Food and Bioproducts Processing, vol. 91, no. 4, pp. 587–594. https://doi.org/10.1016/j.fbp.2013.02.004

Fungal fucoidanase production by solid-state fermentation in a rotating drum bioreactor using algal biomass as substrate. / Rodríguez-Jasso, Rosa M.; Mussatto, Solange I.; Sepúlveda, Leonardo; Torrado-Agrasar, Ana; Pastrana , Lorenzo; Aguilar, Cristobal N.; Teixeira, Jose A. .

In: Food and Bioproducts Processing, Vol. 91, No. 4, 2013, p. 587–594.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Fungal fucoidanase production by solid-state fermentation in a rotating drum bioreactor using algal biomass as substrate

AU - Rodríguez-Jasso, Rosa M.

AU - Mussatto, Solange I.

AU - Sepúlveda, Leonardo

AU - Torrado-Agrasar, Ana

AU - Pastrana , Lorenzo

AU - Aguilar, Cristobal N.

AU - Teixeira, Jose A.

PY - 2013

Y1 - 2013

N2 - Fucoidanase enzymes able to degrade fucoidan were produced by solid-state fermentation (SSF). The fermentation assays were initially carried out in a laboratory-scale rotating drum bioreactor, and two fungal strains (Aspergillus niger PSH and Mucor sp. 3P) and three algal substrates (untreated, autohydrolyzed, and microwave processed seaweed Fucus vesiculosus) were evaluated. Additionally, fermentations were carried out under rotational (10 rpm) and static conditions in order to determine the effect of the agitation on the enzyme production. Agitated experiments showed advantages in the induction of the enzyme when compared to the static ones. The conditions that promoted the maximum fucoidanase activity (3.82 U L−1) consisted in using Mucor sp. 3P as fungal strain, autohydrolyzed alga as substrate, and the rotational system. Such conditions were subsequently used in a 10 times larger scale rotating drum bioreactor. In this step, the effect of controlling the substrate moisture during the enzyme production by SSF was investigated. Moreover, assays combining the algal substrate with an inert support (synthetic fiber) were also carried out. Fermentation of the autohydrolyzed alga with the moisture content maintained at 80% during the fermentation with Mucor sp. 3P gave the highest enzyme activity (9.62 U L−1).

AB - Fucoidanase enzymes able to degrade fucoidan were produced by solid-state fermentation (SSF). The fermentation assays were initially carried out in a laboratory-scale rotating drum bioreactor, and two fungal strains (Aspergillus niger PSH and Mucor sp. 3P) and three algal substrates (untreated, autohydrolyzed, and microwave processed seaweed Fucus vesiculosus) were evaluated. Additionally, fermentations were carried out under rotational (10 rpm) and static conditions in order to determine the effect of the agitation on the enzyme production. Agitated experiments showed advantages in the induction of the enzyme when compared to the static ones. The conditions that promoted the maximum fucoidanase activity (3.82 U L−1) consisted in using Mucor sp. 3P as fungal strain, autohydrolyzed alga as substrate, and the rotational system. Such conditions were subsequently used in a 10 times larger scale rotating drum bioreactor. In this step, the effect of controlling the substrate moisture during the enzyme production by SSF was investigated. Moreover, assays combining the algal substrate with an inert support (synthetic fiber) were also carried out. Fermentation of the autohydrolyzed alga with the moisture content maintained at 80% during the fermentation with Mucor sp. 3P gave the highest enzyme activity (9.62 U L−1).

U2 - 10.1016/j.fbp.2013.02.004

DO - 10.1016/j.fbp.2013.02.004

M3 - Journal article

VL - 91

SP - 587

EP - 594

JO - Food and Bioproducts Processing

JF - Food and Bioproducts Processing

SN - 0960-3085

IS - 4

ER -