Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedings – Annual report year: 2013Researchpeer-review

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We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol−ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic
chip featuring an embedded thiol−ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation
between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic devices, the optical properties of thiol−ene was evaluated by determining the transparency and
refractive index of the cured polymer.
Original languageEnglish
Title of host publicationProceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Publication date2013
Pages745-747
ISBN (Print)978-0-9798064-6-9
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Freiberg, Germany
Duration: 27 Oct 201331 Oct 2013
Conference number: 17
http://www.microtas2013.org/

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Number17
CountryGermany
CityFreiberg
Period27/10/201331/10/2013
Internet address

Bibliographical note

Konferencebidrag 2013

    Research areas

  • Thiol−ene polymers, Biomolecule immobilization, Optofluidic chip

ID: 81271146