Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device

Nikolaj Agentoft Feidenhans'l; Thomas Glasdam Jensen; Josiane P. Lafleur; Jörg Peter Kutter

Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device

Nikolaj Agentoft Feidenhans'l
, Thomas Glasdam Jensen
, Josiane P. Lafleur
, Jörg Peter Kutter

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Abstract

We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol−ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic
chip featuring an embedded thiol−ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation
between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic devices, the optical properties of thiol−ene was evaluated by determining the transparency and
refractive index of the cured polymer.

Original language	English
Title of host publication	Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Publication date	2013
Pages	745-747
ISBN (Print)	978-0-9798064-6-9
Publication status	Published - 2013
Event	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Messe Freiburg, Freiberg, Germany Duration: 27 Oct 2013 → 31 Oct 2013 Conference number: 17 https://microtasconferences.org/microtas2013/

Conference

Conference	17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Number	17
Location	Messe Freiburg
Country/Territory	Germany
City	Freiberg
Period	27/10/2013 → 31/10/2013
Internet address	https://microtasconferences.org/microtas2013/

Bibliographical note

Konferencebidrag 2013

Keywords

Thiol−ene polymers
Biomolecule immobilization
Optofluidic chip

OpenUrl availability

Check availability in DTU Findit

Cite this

Feidenhans'l, N. A., Jensen, T. G., Lafleur, J. P., & Kutter, J. P. (2013). Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device. In Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 745-747). Chemical and Biological Microsystems Society.

Feidenhans'l, Nikolaj Agentoft ; Jensen, Thomas Glasdam ; Lafleur, Josiane P. et al. / Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device. Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2013. pp. 745-747

@inproceedings{5d35d01d28594741a9ae655a96cc5088,

title = "Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device",

abstract = "We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol−ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol−ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic devices, the optical properties of thiol−ene was evaluated by determining the transparency and refractive index of the cured polymer.",

keywords = "Thiol−ene polymers, Biomolecule immobilization, Optofluidic chip",

author = "Feidenhans'l, \{Nikolaj Agentoft\} and Jensen, \{Thomas Glasdam\} and Lafleur, \{Josiane P.\} and Kutter, \{J{\"o}rg Peter\}",

note = "Konferencebidrag 2013; 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",

year = "2013",

language = "English",

isbn = "978-0-9798064-6-9",

pages = "745--747",

booktitle = "Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

url = "https://microtasconferences.org/microtas2013/",

}

Feidenhans'l, NA, Jensen, TG, Lafleur, JP & Kutter, JP 2013, Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device. in Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 745-747, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Freiberg, Baden-Württemberg, Germany, 27/10/2013.

Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device. / Feidenhans'l, Nikolaj Agentoft; Jensen, Thomas Glasdam; Lafleur, Josiane P. et al.
Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2013. p. 745-747.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

TY - GEN

T1 - Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device

AU - Feidenhans'l, Nikolaj Agentoft

AU - Jensen, Thomas Glasdam

AU - Lafleur, Josiane P.

AU - Kutter, Jörg Peter

N1 - Conference code: 17

PY - 2013

Y1 - 2013

N2 - We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol−ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol−ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic devices, the optical properties of thiol−ene was evaluated by determining the transparency and refractive index of the cured polymer.

AB - We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol−ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol−ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic devices, the optical properties of thiol−ene was evaluated by determining the transparency and refractive index of the cured polymer.

KW - Thiol−ene polymers

KW - Biomolecule immobilization

KW - Optofluidic chip

M3 - Article in proceedings

SN - 978-0-9798064-6-9

SP - 745

EP - 747

BT - Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 27 October 2013 through 31 October 2013

ER -

Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device

Abstract

Conference

Bibliographical note

Keywords

OpenUrl availability

Fingerprint

Cite this