Functionalization of embedded thiol-ene waveguides for evanescent wave induced fluorescence detection in a microfluidic device

Nikolaj Agentoft Feidenhans'l, Thomas Glasdam Jensen, Josiane P. Lafleur, Jörg Peter Kutter

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol−ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic
    chip featuring an embedded thiol−ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation
    between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic devices, the optical properties of thiol−ene was evaluated by determining the transparency and
    refractive index of the cured polymer.
    Original languageEnglish
    Title of host publicationProceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherChemical and Biological Microsystems Society
    Publication date2013
    Pages745-747
    ISBN (Print)978-0-9798064-6-9
    Publication statusPublished - 2013
    Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Freiberg, Germany
    Duration: 27 Oct 201331 Oct 2013
    Conference number: 17
    http://www.microtas2013.org/

    Conference

    Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Number17
    CountryGermany
    CityFreiberg
    Period27/10/201331/10/2013
    Internet address

    Bibliographical note

    Konferencebidrag 2013

    Keywords

    • Thiol−ene polymers
    • Biomolecule immobilization
    • Optofluidic chip

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