Abstract
We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol−ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic
chip featuring an embedded thiol−ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation
between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic devices, the optical properties of thiol−ene was evaluated by determining the transparency and
refractive index of the cured polymer.
chip featuring an embedded thiol−ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation
between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol−ene for optofluidic devices, the optical properties of thiol−ene was evaluated by determining the transparency and
refractive index of the cured polymer.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Publication date | 2013 |
| Pages | 745-747 |
| ISBN (Print) | 978-0-9798064-6-9 |
| Publication status | Published - 2013 |
| Event | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Messe Freiburg, Freiberg, Germany Duration: 27 Oct 2013 → 31 Oct 2013 Conference number: 17 https://microtasconferences.org/microtas2013/ |
Conference
| Conference | 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|---|
| Number | 17 |
| Location | Messe Freiburg |
| Country/Territory | Germany |
| City | Freiberg |
| Period | 27/10/2013 → 31/10/2013 |
| Internet address |
Bibliographical note
Konferencebidrag 2013Keywords
- Thiol−ene polymers
- Biomolecule immobilization
- Optofluidic chip