Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome Editing

Jakob Weber, Vito Valiante, Christina Spuur Nødvig, Derek J. Mattern, Rebecca A. Slotkowski, Uffe Hasbro Mortensen, Axel A. Brakhage

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Filamentous fungi produce varieties of natural products even in a strain dependent manner. However, the genetic basis of chemical speciation between strains is still widely unknown. One example is trypacidin, a natural product of the opportunistic human pathogen Aspergillus fumigatus, which is not produced among different isolates. Combining computational analysis with targeted gene editing, we could link a single nucleotide insertion in the polyketide synthase of the trypacidin biosynthetic pathway and reconstitute its production in a nonproducing strain. Thus, we present a CRISPR/Cas9-based tool for advanced molecular genetic studies in filamentous fungi, exploiting selectable markers separated from the edited locus.
    Original languageEnglish
    JournalA C S Synthetic Biology
    Volume6
    Issue number1
    Pages (from-to)62–68
    ISSN2161-5063
    DOIs
    Publication statusPublished - 2017

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