TY - JOUR
T1 - Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome Editing
AU - Weber, Jakob
AU - Valiante, Vito
AU - Nødvig, Christina Spuur
AU - Mattern, Derek J.
AU - Slotkowski, Rebecca A.
AU - Mortensen, Uffe Hasbro
AU - Brakhage, Axel A.
PY - 2017
Y1 - 2017
N2 - Filamentous fungi produce varieties of natural products even in a strain dependent manner. However, the genetic basis of chemical speciation between strains is still widely unknown. One example is trypacidin, a natural product of the opportunistic human pathogen Aspergillus fumigatus, which is not produced among different isolates. Combining computational analysis with targeted gene editing, we could link a single nucleotide insertion in the polyketide synthase of the trypacidin biosynthetic pathway and reconstitute its production in a nonproducing strain. Thus, we present a CRISPR/Cas9-based tool for advanced molecular genetic studies in filamentous fungi, exploiting selectable markers separated from the edited locus.
AB - Filamentous fungi produce varieties of natural products even in a strain dependent manner. However, the genetic basis of chemical speciation between strains is still widely unknown. One example is trypacidin, a natural product of the opportunistic human pathogen Aspergillus fumigatus, which is not produced among different isolates. Combining computational analysis with targeted gene editing, we could link a single nucleotide insertion in the polyketide synthase of the trypacidin biosynthetic pathway and reconstitute its production in a nonproducing strain. Thus, we present a CRISPR/Cas9-based tool for advanced molecular genetic studies in filamentous fungi, exploiting selectable markers separated from the edited locus.
U2 - 10.1021/acssynbio.6b00203
DO - 10.1021/acssynbio.6b00203
M3 - Journal article
C2 - 27611015
SN - 2161-5063
VL - 6
SP - 62
EP - 68
JO - A C S Synthetic Biology
JF - A C S Synthetic Biology
IS - 1
ER -