Functional identification and monitoring of individual alpha and beta cells in cultured mouse islets of langerhans.

Gertrud Malene Hjortø, G.M. Hagel, B.R. Terry, P.O.G. Arkhammar

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The aim of the present study was to evaluate,
by use of fluorescence microscopy and immunofluorescence
stainings, the use of a fluorescent membrane potential
sensitive probe as a means to identify and monitor
changes in membrane potential of individual cell types in
whole islets of Langerhans over time. Our work supports
the use of the fluorescent probe bis-(1,3 dibutylbarbituric
acid) trimethine oxonol (diBAC4(3)), in identification of
single α and β cells in the periphery of mouse pancreatic
islets cultured on extracellular matrix. At a low extracellular
glucose concentration (3 mM), heterogeneous staining
of the islets was observed. Approximately 97% of the
peripheral cells that stained brightly with diBAC4(3)
were glucagon positive. Additional diBAC4(3) studies,
demonstrated that an increase in glucose concentration
from 3 to 10 mM is paralleled by repolarization of α cells
and depolarization of β cells. This suggests that reciprocity
of glucagon and insulin release upon glucose stimulation
is coupled to divergent changes in membrane potential
of these cell types and supports the use of diBAC4(3)
as a means to detect changes in secretion in both cell
types.
Original languageEnglish
JournalActa Diabetologica
Volume41
Pages (from-to)185-193
ISSN0940-5429
Publication statusPublished - 2004
Externally publishedYes

Cite this

Hjortø, G. M., Hagel, G. M., Terry, B. R., & Arkhammar, P. O. G. (2004). Functional identification and monitoring of individual alpha and beta cells in cultured mouse islets of langerhans. Acta Diabetologica, 41, 185-193.