TY - JOUR
T1 - Fully synthetic polymer vesicles for intracellular delivery of antibodies in live cells
AU - Canton, Irene
AU - Massignani, Marzia
AU - Patikarnmonthon, Nisa
AU - Chierico, Luca
AU - Robertson, James
AU - Renshaw, Stephen A.
AU - Warren, Nicholas J.
AU - Jeppe Madsen, Peter
AU - Armes, Steven P.
AU - Lewis, Andrew L.
AU - Battaglia, Giuseppe
PY - 2013
Y1 - 2013
N2 - There is an emerging need both in pharmacology and within the biomedical industry to develop new tools to target intracellular mechanisms. The efficient delivery of functionally active proteins within cells is potentially a powerful research strategy, especially through the use of antibodies. In this work, we report on a nanovector for the efficient encapsulation and delivery of antibodies into live cells with no significant loss of cell viability or any deleterious effect on cell metabolic activity. This delivery system is based on poly[2-(methacryloyloxy) ethyl phosphorylcholine]-block-[2-(diisopropylamino) ethyl methacrylate] (PMPC-PDPA), a pH-sensitive diblock copolymer that self-assembles to form nanometer-sized vesicles, also known as polymersomes, at physiological pH. Polymersomes can successfully deliver relatively high antibody payloads within different types of live cells. We demonstrate that these antibodies can target their respective epitope showing immunolabeling of gamma-tubulin, actin, Golgi protein, and the transcription factor NF-kappa B in live cells. Finally, we demonstrate that intracellular delivery of antibodies can control specific subcellular events, as well as modulate cell activity and proinflammatory processes.-Canton, I., Massignani, M., Patikarnmonthon, N., Chierico, L., Robertson, J., Renshaw, S. A., Warren, N. J., Madsen, J. P., Armes, S P., Lewis, A. L., Battaglia, G. Fully synthetic polymer vesicles for intracellular delivery of antibodies in live cells. FASEB J. 27, 98-108 (2013). www.fasebj.org
AB - There is an emerging need both in pharmacology and within the biomedical industry to develop new tools to target intracellular mechanisms. The efficient delivery of functionally active proteins within cells is potentially a powerful research strategy, especially through the use of antibodies. In this work, we report on a nanovector for the efficient encapsulation and delivery of antibodies into live cells with no significant loss of cell viability or any deleterious effect on cell metabolic activity. This delivery system is based on poly[2-(methacryloyloxy) ethyl phosphorylcholine]-block-[2-(diisopropylamino) ethyl methacrylate] (PMPC-PDPA), a pH-sensitive diblock copolymer that self-assembles to form nanometer-sized vesicles, also known as polymersomes, at physiological pH. Polymersomes can successfully deliver relatively high antibody payloads within different types of live cells. We demonstrate that these antibodies can target their respective epitope showing immunolabeling of gamma-tubulin, actin, Golgi protein, and the transcription factor NF-kappa B in live cells. Finally, we demonstrate that intracellular delivery of antibodies can control specific subcellular events, as well as modulate cell activity and proinflammatory processes.-Canton, I., Massignani, M., Patikarnmonthon, N., Chierico, L., Robertson, J., Renshaw, S. A., Warren, N. J., Madsen, J. P., Armes, S P., Lewis, A. L., Battaglia, G. Fully synthetic polymer vesicles for intracellular delivery of antibodies in live cells. FASEB J. 27, 98-108 (2013). www.fasebj.org
KW - Immunotargeting
KW - Protein
KW - Antibody
KW - Nanovector
U2 - 10.1096/fj.12-212183
DO - 10.1096/fj.12-212183
M3 - Journal article
SN - 0892-6638
VL - 27
SP - 98
EP - 108
JO - FASEB Journal
JF - FASEB Journal
IS - 1
ER -