Full NMR assignment, revised structure and biosynthetic analysis for the capsular polysaccharide from Streptococcus Pneumoniae serotype 15F

Chengxin Li, Kirstine B. Andersen, Pernille L. Elverdal, Ian C. Skovsted, Jens Ø. Duus, Christian Kjeldsen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Upon investigation of Streptococcus pneumoniae serotype 15F capsular polysaccharide (CPS), we discovered that it had a different phosphorylation substituent, namely glycerol-2-phosphate like the other serogroup 15 CPS rather than the originally reported 0.2 equivalent of phosphate or phosphocholine. Furthermore, we also determined the locations of the two previously unassigned O-acetyl groups present in the repeating unit of the 15F CPS, and carried out full NMR assignments of the 15F as well as 15A CPS. Lastly, a biosynthetic analysis of serotypes 15F and 15A was performed and used to make a prediction for the structure of the recently discovered serotype 15D.
Original languageEnglish
Article number108418
JournalCarbohydrate Research
Volume508
Number of pages6
ISSN0008-6215
DOIs
Publication statusPublished - 2021

Keywords

  • Capsular polysaccharide
  • Nuclear magnetic resonance
  • Streptococcus pneumoniae

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