Full genome sequence of a Danish isolate of Mycobacterium avium subspecies paratuberculosis, strain Ejlskov2007

Mamuna Afzal, Soad Abidi, Heidi Mikkelsen, Sheila Tuyet Tang, Ole Lund, Morten Nielsen, Claus Lundegaard, Gregers Jungersen, David Ussery

    Research output: Contribution to conferencePosterResearch

    Abstract

    We have sequenced a Danish isolate of Mycobacterium avium subspecies paratuberculosis, strain Ejlskov2007. The strain was isolated from faecal material of a 48 month old second parity Danish Holstein cow, with clinical symptoms of chronic diarrhoea and emaciation. The cultures were grown on Löwenstein-Jensen media by standard procedures and passed once to new tubes before DNA extraction and being sequenced on a 454 FLX machine. Currently, the genome has been assembled into 70 contiguous pieces, for a total of around 5.0 Mbp, with a 63% GC content. We have predicted a total of 4687 proteins, consisting of 4317 unique gene families. Comparison with M. avium paratuberculosis strain K10 revealed only 3436 genes in common (~70%). We have used GenomeAtlases to show conserved (and unique) regions along the Ejlskov2007 chromosome, compared to 2 other Mycobacterium avium sequenced genomes. Pan-genome analyses of the sequenced Mycobacterium genomes reveal a surprisingly open and diverse set of genes for this bacterial genera.
    Original languageEnglish
    Publication date2009
    Publication statusPublished - 2009
    Event10th International Colloquium on Paratuberculosis - Minneapolis, MN, United States
    Duration: 9 Aug 200913 Aug 2009
    Conference number: 10

    Conference

    Conference10th International Colloquium on Paratuberculosis
    Number10
    CountryUnited States
    CityMinneapolis, MN
    Period09/08/200913/08/2009

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