We have sequenced a Danish isolate of Mycobacterium avium subspecies paratuberculosis, strain Ejlskov2007. The strain was isolated from faecal material of a 48 month old second parity Danish Holstein cow, with clinical symptoms of chronic diarrhoea and emaciation. The cultures were grown on Löwenstein-Jensen media by standard procedures and passed once to new tubes before DNA extraction and being sequenced on a 454 FLX machine. Currently, the genome has been assembled into 70 contiguous pieces, for a total of around 5.0 Mbp, with a 63% GC content. We have predicted a total of 4687 proteins, consisting of 4317 unique gene families. Comparison with M. avium paratuberculosis strain K10 revealed only 3436 genes in common (~70%). We have used GenomeAtlases to show conserved (and unique) regions along the Ejlskov2007 chromosome, compared to 2 other Mycobacterium avium sequenced genomes. Pan-genome analyses of the sequenced Mycobacterium genomes reveal a surprisingly open and diverse set of genes for this bacterial genera.
|Publication status||Published - 2009|
|Event||10th International Colloquium on Paratuberculosis - Minneapolis, MN, United States|
Duration: 9 Aug 2009 → 13 Aug 2009
Conference number: 10
|Conference||10th International Colloquium on Paratuberculosis|
|Period||09/08/2009 → 13/08/2009|