Front line defenders of the ecological niche! Screening the structural diversity of peptaibiotics from saprotrophic and fungicolous Trichoderma/Hypocrea species

Christian René Röhrich, Walter Michael Jaklitsch, Hermann Voglmayr, Anita Iversen, Andreas Vilcinskas, Kristian Fog Nielsen, Ulf Thrane, Hans von Döhren, Hans Brückner, Thomas Degenkolb

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    Abstract

    Approximately 950 individual sequences of nonribosomally biosynthesised peptides are produced by the genus Trichoderma/Hypocreathat belong to a perpetually growing class of mostly linear antibiotic oligopeptides, which are rich in the non-proteinogenic α-aminoisobutyric acid (Aib). Thus, they are
    comprehensively named peptaibiotics. Notably, peptaibiotics represent ca. 80%of the total inventory of secondary metabolites currently known from Trichoderma/Hypocrea. Their unique membrane-modifying bioactivity results from amphipathicity and helicity, thus making them ideal candidates in assisting both colonisation and defence of the natural habitats by their fungal producers. Despite this, reports on the in vivo-detection of peptaibiotics have scarcely been published in the past. In order to evaluate the significance of peptaibiotic production for a broader range of potential producers, we screened nine specimens
    belonging to seven hitherto uninvestigated fungicolous or
    saprotrophic Trichoderma/Hypocrea species by liquid chromatography
    coupled to electrospray high resolution mass spectrometry.
    Sequences of peptaibiotics found were independently confirmed
    by analysing the peptaibiome of pure agar cultures
    Original languageEnglish
    JournalFungal Diversity
    Volume69
    Issue number1
    Pages (from-to)117-146
    Number of pages30
    ISSN1560-2745
    DOIs
    Publication statusPublished - 2014

    Bibliographical note

    Available under Open Access

    Keywords

    • HPLC/QTOF-ESI-HRMS
    • Metabolite profiling
    • Peptaibiotics
    • Peptaibols
    • Aib peptides
    • Trichoderma
    • Hypocrea

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