From DNA to biomass: opportunities and challenges in species quantification of bulk fisheries products

Brian Klitgaard Hansen*, Gregory Kevin Farrant, Rob Ogden, Emily Humble, Guðbjörg Ólafsdóttir, Dorte Bekkevold, Steen Wilhelm Knudsen, Peter Rask Møller, Einar Eg Nielsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Fisheries enforcement relies on visual catch identification and quantification at sea or when landed. Silage (fish dissolved in acid) and fish blocks (block frozen fish) are promising methods for on-board processing and storage of low-value catches. We examined the use of nondestructive sampling and two DNA-based methods, quantitative PCR (qPCR) and metabarcoding, to assess species composition and relative abundance in industrial grade experimental silage and fish blocks. We demonstrate the ability to identify and quantify DNA from fish species in both products. qPCR analysis of small silage samples collected over 21 days detected all target control species. DNA from one species (Atlantic wolffish) was consistently overrepresented while, for three species of gadoids (Atlantic cod, haddock and whiting), the DNA content matched input tissue proportions with high accuracy. qPCR and metabarcoding of fish blocks, sampled as run-off water and exterior swabs, provided consistent species detection, with the highest variance observed in quantification from swab samples. Our analysis shows that DNA-based methods have significant potential as a tool for species identification and quantification of complex on-board-processed seafood products and are readily applicable to taxonomically and morphologically similar fish. There is, however, a need for establishing DNA/weight calibration factors for primary fisheries species.
Original languageEnglish
Article numberfsaa115
JournalICES Journal of Marine Science
Volume77
Issue number7-8
Pages (from-to)2557-2566
Number of pages10
ISSN1054-3139
DOIs
Publication statusPublished - 2020

Keywords

  • DNA quantification
  • Metabarcoding
  • MinION
  • Multi-species fisheries products
  • Quantitative PCR

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