From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis

Mohammad-Ali Shahbazi; Krishna Kant; Joseph John Kaplinsky; Vinayaka Aaydha Chidambara; Dang Duong Bang; Anders Wolff

doi:10.1109/MEMSYS.2017.7863437

From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis

Mohammad-Ali Shahbazi, Krishna Kant, Joseph John Kaplinsky, Vinayaka Aaydha Chidambara, Dang Duong Bang, Anders Wolff

National Food Institute

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Abstract

A combined 2D microfluidic-microarray high throughput approach is reported to identify universal bacterial capturing ligands that can be tethered on the surface of 3D sponges fabricated by different methods for concentrating of bacterial targets in diagnosis devices. The developed platform allows for the first time the simultaneous monitoring of various ligands' affinities to different bacteria species in a dynamic condition in vitro. Moreover, it has been feasible to recognize the effect of steric hindrance on the function of capturing motifs through immobilizing spacer molecules with different lengths between the solid surface and ligands. 3D sponges and micropillars are modified with the most potent capturing molecule to assess their bacterial capturing in real blood samples. Next, the 3D structures are placed into a chip with an immense potential to recognize bacteria through imaging and fluorescence intensity concept.

Original language	English
Title of host publication	Proceedings of 30th international conference on micro electro mechanical systems (MEMS)
Number of pages	4
Publisher	IEEE
Publication date	2017
Pages	440-443
ISBN (Print)	978-1-5090-5079-6
ISBN (Electronic)	978-1-5090-5078-9
DOIs	https://doi.org/10.1109/MEMSYS.2017.7863437
Publication status	Published - 2017
Event	2017 IEEE 30th International Conference on Micro Electro Mechanical Systems - Las Vegas, United States Duration: 22 Jan 2017 → 26 Jan 2017 Conference number: 30 https://ieeexplore.ieee.org/xpl/conhome/7852393/proceeding

Conference

Conference	2017 IEEE 30th International Conference on Micro Electro Mechanical Systems
Number	30
Country/Territory	United States
City	Las Vegas
Period	22/01/2017 → 26/01/2017
Internet address	https://ieeexplore.ieee.org/xpl/conhome/7852393/proceeding

Keywords

microorganisms
biomedical imaging
bioMEMS
bio-optics
blood
lab-on-a-chip
microfluidics
fluorescence intensity
2D fluidic array screening
3D bacterial capturing structures
point of care system
sepsis diagnosis
2D microfluidic-microarray high throughput approach
bacterial capturing ligands
3D sponges
diagnosis devices
steric hindrance
capturing motifs
spacer molecule immobilization
micropillars
real blood samples
imaging
Microorganisms
Surface treatment
Three-dimensional displays
Surface morphology
Substrates
Two dimensional displays
Peptides
Patient diagnostic methods and instrumentation
Micromechanical and nanomechanical devices and systems
Applied fluid mechanics
Microfluidics and nanofluidics
Optical and laser radiation (medical uses)
Optical and laser radiation (biomedical imaging/measurement)
MEMS and NEMS device technology

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10.1109/MEMSYS.2017.7863437

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Shahbazi, M.-A., Kant, K., Kaplinsky, J. J., Aaydha Chidambara, V., Bang, D. D., & Wolff, A. (2017). From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. In Proceedings of 30th international conference on micro electro mechanical systems (MEMS) (pp. 440-443). IEEE. https://doi.org/10.1109/MEMSYS.2017.7863437

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year = "2017",

doi = "10.1109/MEMSYS.2017.7863437",

language = "English",

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booktitle = "Proceedings of 30th international conference on micro electro mechanical systems (MEMS)",

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note = "2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, MEMS 2017 ; Conference date: 22-01-2017 Through 26-01-2017",

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Shahbazi, M-A, Kant, K, Kaplinsky, JJ, Aaydha Chidambara, V, Bang, DD & Wolff, A 2017, From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. in Proceedings of 30th international conference on micro electro mechanical systems (MEMS). IEEE, pp. 440-443, 2017 IEEE 30th International Conference on Micro Electro Mechanical Systems, Las Vegas, Nevada, United States, 22/01/2017. https://doi.org/10.1109/MEMSYS.2017.7863437

From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. / Shahbazi, Mohammad-Ali; Kant, Krishna; Kaplinsky, Joseph John et al.
Proceedings of 30th international conference on micro electro mechanical systems (MEMS). IEEE, 2017. p. 440-443.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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ER -

From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis

Abstract

Conference

Keywords

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