From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis

Mohammad-Ali Shahbazi, Krishna Kant, Joseph John Kaplinsky, Vinayaka Aaydha Chidambara, Dang Duong Bang, Anders Wolff

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Abstract

A combined 2D microfluidic-microarray high throughput approach is reported to identify universal bacterial capturing ligands that can be tethered on the surface of 3D sponges fabricated by different methods for concentrating of bacterial targets in diagnosis devices. The developed platform allows for the first time the simultaneous monitoring of various ligands' affinities to different bacteria species in a dynamic condition in vitro. Moreover, it has been feasible to recognize the effect of steric hindrance on the function of capturing motifs through immobilizing spacer molecules with different lengths between the solid surface and ligands. 3D sponges and micropillars are modified with the most potent capturing molecule to assess their bacterial capturing in real blood samples. Next, the 3D structures are placed into a chip with an immense potential to recognize bacteria through imaging and fluorescence intensity concept.
Original languageEnglish
Title of host publicationProceedings of 30th international conference on micro electro mechanical systems (MEMS)
Number of pages4
PublisherIEEE
Publication date2017
Pages440-443
ISBN (Print)978-1-5090-5079-6
ISBN (Electronic)978-1-5090-5078-9
DOIs
Publication statusPublished - 2017
Event30th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2017) - Berkeley, United States
Duration: 22 Jan 201726 Jan 2017
Conference number: 30

Conference

Conference30th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2017)
Number30
CountryUnited States
CityBerkeley
Period22/01/201726/01/2017
Series2017 Ieee 30th International Conference on Micro-electro-mechanical Systems (mems)

Keywords

  • microorganisms
  • biomedical imaging
  • bioMEMS
  • bio-optics
  • blood
  • lab-on-a-chip
  • microfluidics
  • fluorescence intensity
  • 2D fluidic array screening
  • 3D bacterial capturing structures
  • point of care system
  • sepsis diagnosis
  • 2D microfluidic-microarray high throughput approach
  • bacterial capturing ligands
  • 3D sponges
  • diagnosis devices
  • steric hindrance
  • capturing motifs
  • spacer molecule immobilization
  • micropillars
  • real blood samples
  • imaging
  • Microorganisms
  • Surface treatment
  • Three-dimensional displays
  • Surface morphology
  • Substrates
  • Two dimensional displays
  • Peptides
  • Patient diagnostic methods and instrumentation
  • Micromechanical and nanomechanical devices and systems
  • Applied fluid mechanics
  • Microfluidics and nanofluidics
  • Optical and laser radiation (medical uses)
  • Optical and laser radiation (biomedical imaging/measurement)
  • MEMS and NEMS device technology

Cite this

Shahbazi, M-A., Kant, K., Kaplinsky, J. J., Aaydha Chidambara, V., Bang, D. D., & Wolff, A. (2017). From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. In Proceedings of 30th international conference on micro electro mechanical systems (MEMS) (pp. 440-443). IEEE. 2017 Ieee 30th International Conference on Micro-electro-mechanical Systems (mems) https://doi.org/10.1109/MEMSYS.2017.7863437
Shahbazi, Mohammad-Ali ; Kant, Krishna ; Kaplinsky, Joseph John ; Aaydha Chidambara, Vinayaka ; Bang, Dang Duong ; Wolff, Anders. / From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. Proceedings of 30th international conference on micro electro mechanical systems (MEMS). IEEE, 2017. pp. 440-443 (2017 Ieee 30th International Conference on Micro-electro-mechanical Systems (mems)).
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title = "From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis",
abstract = "A combined 2D microfluidic-microarray high throughput approach is reported to identify universal bacterial capturing ligands that can be tethered on the surface of 3D sponges fabricated by different methods for concentrating of bacterial targets in diagnosis devices. The developed platform allows for the first time the simultaneous monitoring of various ligands' affinities to different bacteria species in a dynamic condition in vitro. Moreover, it has been feasible to recognize the effect of steric hindrance on the function of capturing motifs through immobilizing spacer molecules with different lengths between the solid surface and ligands. 3D sponges and micropillars are modified with the most potent capturing molecule to assess their bacterial capturing in real blood samples. Next, the 3D structures are placed into a chip with an immense potential to recognize bacteria through imaging and fluorescence intensity concept.",
keywords = "microorganisms, biomedical imaging, bioMEMS, bio-optics, blood, lab-on-a-chip, microfluidics, fluorescence intensity, 2D fluidic array screening, 3D bacterial capturing structures, point of care system, sepsis diagnosis, 2D microfluidic-microarray high throughput approach, bacterial capturing ligands, 3D sponges, diagnosis devices, steric hindrance, capturing motifs, spacer molecule immobilization, micropillars, real blood samples, imaging, Microorganisms, Surface treatment, Three-dimensional displays, Surface morphology, Substrates, Two dimensional displays, Peptides, Patient diagnostic methods and instrumentation, Micromechanical and nanomechanical devices and systems, Applied fluid mechanics, Microfluidics and nanofluidics, Optical and laser radiation (medical uses), Optical and laser radiation (biomedical imaging/measurement), MEMS and NEMS device technology",
author = "Mohammad-Ali Shahbazi and Krishna Kant and Kaplinsky, {Joseph John} and {Aaydha Chidambara}, Vinayaka and Bang, {Dang Duong} and Anders Wolff",
year = "2017",
doi = "10.1109/MEMSYS.2017.7863437",
language = "English",
isbn = "978-1-5090-5079-6",
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Shahbazi, M-A, Kant, K, Kaplinsky, JJ, Aaydha Chidambara, V, Bang, DD & Wolff, A 2017, From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. in Proceedings of 30th international conference on micro electro mechanical systems (MEMS). IEEE, 2017 Ieee 30th International Conference on Micro-electro-mechanical Systems (mems), pp. 440-443, 30th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2017), Berkeley, United States, 22/01/2017. https://doi.org/10.1109/MEMSYS.2017.7863437

From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. / Shahbazi, Mohammad-Ali; Kant, Krishna; Kaplinsky, Joseph John; Aaydha Chidambara, Vinayaka; Bang, Dang Duong; Wolff, Anders.

Proceedings of 30th international conference on micro electro mechanical systems (MEMS). IEEE, 2017. p. 440-443 (2017 Ieee 30th International Conference on Micro-electro-mechanical Systems (mems)).

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

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AB - A combined 2D microfluidic-microarray high throughput approach is reported to identify universal bacterial capturing ligands that can be tethered on the surface of 3D sponges fabricated by different methods for concentrating of bacterial targets in diagnosis devices. The developed platform allows for the first time the simultaneous monitoring of various ligands' affinities to different bacteria species in a dynamic condition in vitro. Moreover, it has been feasible to recognize the effect of steric hindrance on the function of capturing motifs through immobilizing spacer molecules with different lengths between the solid surface and ligands. 3D sponges and micropillars are modified with the most potent capturing molecule to assess their bacterial capturing in real blood samples. Next, the 3D structures are placed into a chip with an immense potential to recognize bacteria through imaging and fluorescence intensity concept.

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KW - Peptides

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KW - Applied fluid mechanics

KW - Microfluidics and nanofluidics

KW - Optical and laser radiation (medical uses)

KW - Optical and laser radiation (biomedical imaging/measurement)

KW - MEMS and NEMS device technology

U2 - 10.1109/MEMSYS.2017.7863437

DO - 10.1109/MEMSYS.2017.7863437

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Shahbazi M-A, Kant K, Kaplinsky JJ, Aaydha Chidambara V, Bang DD, Wolff A. From 2D fluidic array screening to 3D bacterial capturing structures in a point of care system for sepsis diagnosis. In Proceedings of 30th international conference on micro electro mechanical systems (MEMS). IEEE. 2017. p. 440-443. (2017 Ieee 30th International Conference on Micro-electro-mechanical Systems (mems)). https://doi.org/10.1109/MEMSYS.2017.7863437