Formation of water-soluble soybean polysaccharides from spent flakes by hydrogen peroxide treatment

Brian Pierce, Jesper Wichmann, Tam H. Tran, Roshan Cheetamun, Antony Bacic, Anne S. Meyer

Research output: Contribution to journalJournal articleResearchpeer-review


In this paper we propose a novel chemical process for the generation of water-soluble polysaccharides from soy spent flake, a by-product of the soy food industry. This process entails treatment of spent flake with hydrogen peroxide at an elevated temperature, resulting in the release of more than 70% of the original insoluble material as high molar mass soluble polysaccharides. A design of experiment was used to quantify the effects of pH, reaction time, and hydrogen peroxide concentration on the reaction yield, average molar mass, and free monosaccharides generated. The resulting product is low in protein, fat, and minerals and contains predominantly water-soluble polysaccharides of high molar mass, including arabinan, type I arabinogalactan, homogalacturonan, xyloglucan, rhamnogalacturonan, and (glucurono)arabinoxylan. This treatment provides a straightforward approach for generation of soluble soy polysaccharides and opens a new range of opportunities for this abundant and underutilized material in future research and industrial applications.
Original languageEnglish
JournalCarbohydrate Polymers
Pages (from-to)504-513
Publication statusPublished - 2016


  • Hydrogen peroxide
  • Soluble fiber
  • Soy polysaccharides
  • Spent flakes

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