Following the Dynamics of pH in Endosomes of Live Cells with SERS Nanosensors

J. Kneipp; Harald Kneipp; B. Wittig; Katrin Kneipp

doi:10.1021/jp910034z

Following the Dynamics of pH in Endosomes of Live Cells with SERS Nanosensors

J. Kneipp
, Harald Kneipp
, B. Wittig
, Katrin Kneipp

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold nanaoaggregates and 4-mercaptobenzoic acid (pMBA) attached as a reporter for monitoring changes in local pH of the cellular compartments of living NIH/3T3 cells. We show that SERS nanosensors enable the dynamics of local pH in individual live cells to be followed at subendosomal resolution in a timeline of cellular processes. This information is of basic interest for a better understanding of a broad range of physiological and metabolic processes as well as for a number of biotechnological applications.

Original language	English
Journal	Journal of Physical Chemistry Part C: Nanomaterials and Interfaces
Volume	114
Issue number	16
Pages (from-to)	7421-7426
ISSN	1932-7447
DOIs	https://doi.org/10.1021/jp910034z
Publication status	Published - 2010

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title = "Following the Dynamics of pH in Endosomes of Live Cells with SERS Nanosensors",

abstract = "The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold nanaoaggregates and 4-mercaptobenzoic acid (pMBA) attached as a reporter for monitoring changes in local pH of the cellular compartments of living NIH/3T3 cells. We show that SERS nanosensors enable the dynamics of local pH in individual live cells to be followed at subendosomal resolution in a timeline of cellular processes. This information is of basic interest for a better understanding of a broad range of physiological and metabolic processes as well as for a number of biotechnological applications.",

author = "J. Kneipp and Harald Kneipp and B. Wittig and Katrin Kneipp",

year = "2010",

doi = "10.1021/jp910034z",

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T1 - Following the Dynamics of pH in Endosomes of Live Cells with SERS Nanosensors

AU - Kneipp, J.

AU - Kneipp, Harald

AU - Wittig, B.

AU - Kneipp, Katrin

PY - 2010

Y1 - 2010

N2 - The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold nanaoaggregates and 4-mercaptobenzoic acid (pMBA) attached as a reporter for monitoring changes in local pH of the cellular compartments of living NIH/3T3 cells. We show that SERS nanosensors enable the dynamics of local pH in individual live cells to be followed at subendosomal resolution in a timeline of cellular processes. This information is of basic interest for a better understanding of a broad range of physiological and metabolic processes as well as for a number of biotechnological applications.

AB - The surface enhanced Raman scattering (SERS) spectrum of a reporter molecule attached to gold or silver nanostructures, which is pH-sensitive, can deliver information on the local pH in the environment of the nanostructure. Here, we demonstrate the use of a mobile SERS nanosensor made from gold nanaoaggregates and 4-mercaptobenzoic acid (pMBA) attached as a reporter for monitoring changes in local pH of the cellular compartments of living NIH/3T3 cells. We show that SERS nanosensors enable the dynamics of local pH in individual live cells to be followed at subendosomal resolution in a timeline of cellular processes. This information is of basic interest for a better understanding of a broad range of physiological and metabolic processes as well as for a number of biotechnological applications.

U2 - 10.1021/jp910034z

DO - 10.1021/jp910034z

M3 - Journal article

SN - 1932-7447

VL - 114

SP - 7421

EP - 7426

JO - Journal of Physical Chemistry Part C: Nanomaterials and Interfaces

JF - Journal of Physical Chemistry Part C: Nanomaterials and Interfaces

IS - 16

ER -

Following the Dynamics of pH in Endosomes of Live Cells with SERS Nanosensors

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