Fluorescence spectroscopy and multi-way techniques. PARAFAC

Kathleen R. Murphy, Colin A. Stedmon, Daniel Graeber, Rasmus Bro

Research output: Contribution to journalJournal articleResearchpeer-review

825 Downloads (Pure)

Abstract

PARAllel FACtor analysis (PARAFAC) is increasingly used to decompose fluorescence excitation emission matrices (EEMs) into their underlying chemical components. In the ideal case where fluorescence conforms to Beers Law, this process can lead to the mathematical identification and quantification of independently varying fluorophores. However, many practical and analytical hurdles stand between EEM datasets and their chemical interpretation. This article provides a tutorial in the practical application of PARAFAC to fluorescence datasets, demonstrated using a dissolved organic matter (DOM) fluorescence dataset. A new toolbox for MATLAB is presented to support improved visualisation and sensitivity analyses of PARAFAC models in fluorescence spectroscopy. © 2013 The Royal Society of Chemistry.
Original languageEnglish
JournalAnalytical Methods
Volume5
Issue number23
Pages (from-to)6557-6566
ISSN1759-9660
DOIs
Publication statusPublished - 2013

Cite this