Fluorescence enhancement of single DNA molecules confined in Si/SiO2 nanochannels

F. Westerlund; Karl Fredrik Persson; Anders Kristensen; J.O. Tegenfeldt

doi:10.1039/c004878j

Fluorescence enhancement of single DNA molecules confined in Si/SiO2 nanochannels

F. Westerlund, Karl Fredrik Persson, Anders Kristensen, J.O. Tegenfeldt

University of Gothenburg

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nano-funnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

Original language	English
Journal	Lab on a Chip
Volume	10
Issue number	16
Pages (from-to)	2049-2051
ISSN	1473-0197
DOIs	https://doi.org/10.1039/c004878j
Publication status	Published - 2010

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title = "Fluorescence enhancement of single DNA molecules confined in Si/SiO2 nanochannels",

abstract = "We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nano-funnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.",

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AU - Persson, Karl Fredrik

AU - Kristensen, Anders

AU - Tegenfeldt, J.O.

PY - 2010

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AB - We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nano-funnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

U2 - 10.1039/c004878j

DO - 10.1039/c004878j

M3 - Journal article

C2 - 20544105

SN - 1473-0197

VL - 10

SP - 2049

EP - 2051

JO - Lab on a Chip

JF - Lab on a Chip

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ER -

Fluorescence enhancement of single DNA molecules confined in Si/SiO2 nanochannels

Abstract

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