We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nano-funnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.
Westerlund, F., Persson, K. F., Kristensen, A., & Tegenfeldt, J. O. (2010). Fluorescence enhancement of single DNA molecules confined in Si/SiO2 nanochannels. Lab on a Chip, 10(16), 2049-2051. https://doi.org/10.1039/c004878j