Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes

Anders Okholm, Jørgen Kjems, Kira Astakhova

Research output: Contribution to journalJournal articleResearchpeer-review

27 Downloads (Pure)

Abstract

Herein a reliable approach to the design of effective fluorescent probes for RNA detection is described. The fluorescence signalling of hybridization by internally positioned polyaromatic hydrocarbons and rhodamine dyes was achieved with a low fluorescence background signal, high fluorescence quantum yields at ambient and elevated temperature, high selectivity and signal specificity of the probes when binding to miR-7 and circRNA targets.
Original languageEnglish
JournalR S C Advances
Volume4
Issue number86
Pages (from-to)45653-45656
Number of pages4
ISSN2046-2069
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Dive into the research topics of 'Fluorescence detection of natural RNA using rationally designed "clickable" oligonucleotide probes'. Together they form a unique fingerprint.

Cite this