Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices

Tanya Bakmand, Ane R. Troels-Smith, Maria Dimaki, Jakob D. Nissen, Karsten Brandt Andersen, Luigi Sasso, Helle S. Waagepetersen, Jan B. Gramsbergen, Winnie Edith Svendsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.
    Original languageEnglish
    Article number71
    JournalBiomedical Microdevices
    Volume17
    Issue number4
    Number of pages7
    ISSN1387-2176
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Fluidic
    • Hippocampus
    • Interface culturing
    • Tissue culturing
    • Brain
    • Electrophysiology
    • Fluidics
    • Brain development
    • Continuous flows
    • Glucose concentration
    • Hippocampal slice
    • Neurodegeneration
    • Nutrient distributions
    • Standard interface
    • Tissue
    • HASH(0x4f00450)

    Cite this

    Bakmand, Tanya ; Troels-Smith, Ane R. ; Dimaki, Maria ; Nissen, Jakob D. ; Andersen, Karsten Brandt ; Sasso, Luigi ; Waagepetersen, Helle S. ; Gramsbergen, Jan B. ; Svendsen, Winnie Edith. / Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices. In: Biomedical Microdevices. 2015 ; Vol. 17, No. 4.
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    title = "Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices",
    abstract = "Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.",
    keywords = "Fluidic, Hippocampus, Interface culturing, Tissue culturing, Brain, Electrophysiology, Fluidics, Brain development, Continuous flows, Glucose concentration, Hippocampal slice, Neurodegeneration, Nutrient distributions, Standard interface, Tissue, HASH(0x4f00450)",
    author = "Tanya Bakmand and Troels-Smith, {Ane R.} and Maria Dimaki and Nissen, {Jakob D.} and Andersen, {Karsten Brandt} and Luigi Sasso and Waagepetersen, {Helle S.} and Gramsbergen, {Jan B.} and Svendsen, {Winnie Edith}",
    year = "2015",
    doi = "10.1007/s10544-015-9973-6",
    language = "English",
    volume = "17",
    journal = "Biomedical Microdevices",
    issn = "1387-2176",
    publisher = "Springer New York",
    number = "4",

    }

    Bakmand, T, Troels-Smith, AR, Dimaki, M, Nissen, JD, Andersen, KB, Sasso, L, Waagepetersen, HS, Gramsbergen, JB & Svendsen, WE 2015, 'Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices', Biomedical Microdevices, vol. 17, no. 4, 71. https://doi.org/10.1007/s10544-015-9973-6

    Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices. / Bakmand, Tanya; Troels-Smith, Ane R.; Dimaki, Maria; Nissen, Jakob D.; Andersen, Karsten Brandt; Sasso, Luigi; Waagepetersen, Helle S.; Gramsbergen, Jan B.; Svendsen, Winnie Edith.

    In: Biomedical Microdevices, Vol. 17, No. 4, 71, 2015.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices

    AU - Bakmand, Tanya

    AU - Troels-Smith, Ane R.

    AU - Dimaki, Maria

    AU - Nissen, Jakob D.

    AU - Andersen, Karsten Brandt

    AU - Sasso, Luigi

    AU - Waagepetersen, Helle S.

    AU - Gramsbergen, Jan B.

    AU - Svendsen, Winnie Edith

    PY - 2015

    Y1 - 2015

    N2 - Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.

    AB - Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.

    KW - Fluidic

    KW - Hippocampus

    KW - Interface culturing

    KW - Tissue culturing

    KW - Brain

    KW - Electrophysiology

    KW - Fluidics

    KW - Brain development

    KW - Continuous flows

    KW - Glucose concentration

    KW - Hippocampal slice

    KW - Neurodegeneration

    KW - Nutrient distributions

    KW - Standard interface

    KW - Tissue

    KW - HASH(0x4f00450)

    U2 - 10.1007/s10544-015-9973-6

    DO - 10.1007/s10544-015-9973-6

    M3 - Journal article

    VL - 17

    JO - Biomedical Microdevices

    JF - Biomedical Microdevices

    SN - 1387-2176

    IS - 4

    M1 - 71

    ER -