Fluidic resistance control enables high-throughput establishment of mixed-species biofilms

Mads Frederik Hansen, Anders Meyer Torp, Jonas Stenløkke Madsen, Henriette Lyng Røder*, Mette Burmølle

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Bacteria often live in communities of mixed species embedded in a self-produced extracellular matrix of polysaccharides, proteins and DNA, termed biofilms. The BioFlux microfluidic flow system is useful for studying biofilm formation in different media under flow. However, analyzing the architecture and maturation of biofilms under flow requires a proper seeding, which can prove difficult when working with bacteria of different sizes, motile bacteria or aiming for a high number of replicates. Here we developed an efficient protocol that exploits viscosity tuning and seeding indicator dyes to improve seeding and allow for high-throughput examination and visualization of consistent mono- and mixed-species biofilm developments under flow.
Original languageEnglish
JournalBiotechniques
Volume66
Issue number5
Pages (from-to)235-239
ISSN0736-6205
DOIs
Publication statusPublished - 2019

Keywords

  • BioFlux
  • CLSM
  • Biofilm analysis
  • Biofilm device
  • Biofilm formation
  • Biofilm seeding
  • Microfluidic device
  • Microfluidic flow systems
  • Mixed species biofilm
  • Shear stress

Cite this

Hansen, Mads Frederik ; Meyer Torp, Anders ; Madsen, Jonas Stenløkke ; Røder, Henriette Lyng ; Burmølle, Mette. / Fluidic resistance control enables high-throughput establishment of mixed-species biofilms. In: Biotechniques. 2019 ; Vol. 66, No. 5. pp. 235-239.
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abstract = "Bacteria often live in communities of mixed species embedded in a self-produced extracellular matrix of polysaccharides, proteins and DNA, termed biofilms. The BioFlux microfluidic flow system is useful for studying biofilm formation in different media under flow. However, analyzing the architecture and maturation of biofilms under flow requires a proper seeding, which can prove difficult when working with bacteria of different sizes, motile bacteria or aiming for a high number of replicates. Here we developed an efficient protocol that exploits viscosity tuning and seeding indicator dyes to improve seeding and allow for high-throughput examination and visualization of consistent mono- and mixed-species biofilm developments under flow.",
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Fluidic resistance control enables high-throughput establishment of mixed-species biofilms. / Hansen, Mads Frederik; Meyer Torp, Anders; Madsen, Jonas Stenløkke; Røder, Henriette Lyng; Burmølle, Mette.

In: Biotechniques, Vol. 66, No. 5, 2019, p. 235-239.

Research output: Contribution to journalJournal articleResearchpeer-review

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T1 - Fluidic resistance control enables high-throughput establishment of mixed-species biofilms

AU - Hansen, Mads Frederik

AU - Meyer Torp, Anders

AU - Madsen, Jonas Stenløkke

AU - Røder, Henriette Lyng

AU - Burmølle, Mette

PY - 2019

Y1 - 2019

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KW - CLSM

KW - Biofilm analysis

KW - Biofilm device

KW - Biofilm formation

KW - Biofilm seeding

KW - Microfluidic device

KW - Microfluidic flow systems

KW - Mixed species biofilm

KW - Shear stress

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