Fluidic automation of nitrate and nitrite bioassays in whole blood by dissolvable-film based centrifugo-pneumatic actuation

Charles E. Nwankire, Di-Sien S. Chan, Jennifer Gaughran, Robert Burger, Robert Gorkin III, Jens Ducrée

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    Abstract

    This paper demonstrates the full centrifugal microfluidic integration and automation of all liquid handling steps of a 7-step fluorescence-linked immunosorbent assay (FLISA) for quantifying nitrate and nitrite levels in whole blood within about 15 min. The assay protocol encompasses the extraction of metered plasma, the controlled release of sample and reagents (enzymes, co-factors and fluorescent labels), and incubation and detection steps. Flow control is implemented by a rotationally actuated dissolvable film (DF) valving scheme. In the valves, the burst pressure is primarily determined by the radial position, geometry and volume of the valve chamber and its inlet channel and can thus be individually tuned over an extraordinarily wide range of equivalent spin rates between 1,000 RPM and 5,500 RPM. Furthermore, the vapour barrier properties of the DF valves are investigated in this paper in order to further show the potential for commercially relevant on-board storage of liquid reagents during shelf-life of bioanalytical, ready-to-use discs. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
    Original languageEnglish
    JournalSensors
    Volume13
    Issue number9
    Pages (from-to)11336-11349
    ISSN1424-8220
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Automation
    • Blood
    • Building materials
    • Centrifugation
    • Liquids
    • Magnetic storage
    • Microfluidics
    • Nitrates
    • Fluorescence

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