Flow manipulation and cell immobilization for biochemical applications using thermally responsive fluids

Anil Haraksingh Thilsted, Vahid Bazargan, Nina Piggott, Vivien Measday, Boris Stoeber

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Abstract

A flow redirection and single cell immobilization method in a microfluidic chip is presented. Microheaters generated localized heating and induced poly(N-isopropylacrylamide) phase transition, creating a hydrogel that blocked a channel or immobilized a single cell. The heaters were activated in sets to redirect flow and exchange the fluid in which an immobilized cell was immersed. A yeast cell was immobilized in hydrogel and a 4',6-diamidino-2-phenylindole (DAPI) fluorescent stain was introduced using flow redirection. DAPI diffused through the hydrogel and fluorescently labelled the yeast DNA, demonstrating in situ single cell biochemistry by means of immobilization and fluid exchange.

Original languageEnglish
JournalBiomicrofluidics
Volume6
Issue number4
Pages (from-to)041101
ISSN1932-1058
DOIs
Publication statusPublished - 2012
Externally publishedYes

Bibliographical note

© 2012 American Institute of Physics

Keywords

  • biochemical application
  • fluid exchange
  • in situ single cell biochemistry
  • Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi [15000] yeast common
  • 4',6-diamidino-2-phenylindole DAPI fluorescent stain
  • DNA
  • hydrogel 124586-38-5
  • thermally responsive fluid
  • 02502, Cytology - General
  • 02504, Cytology - Plant
  • 10060, Biochemistry studies - General
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10511, Biophysics - Bioengineering
  • 51522, Plant physiology - Chemical constituents
  • cell immobilization laboratory techniques
  • flow manipulation laboratory techniques
  • flow redirection laboratory techniques
  • Biochemistry and Molecular Biophysics
  • Biomaterials
  • Cell Biology
  • Methods and Techniques

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