Flow manipulation and cell immobilization for biochemical applications using thermally responsive fluids

Anil Haraksingh Thilsted; Vahid Bazargan; Nina Piggott; Vivien Measday; Boris Stoeber

doi:10.1063/1.4768905

Flow manipulation and cell immobilization for biochemical applications using thermally responsive fluids

Anil Haraksingh Thilsted, Vahid Bazargan, Nina Piggott, Vivien Measday, Boris Stoeber

University of British Columbia

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Abstract

A flow redirection and single cell immobilization method in a microfluidic chip is presented. Microheaters generated localized heating and induced poly(N-isopropylacrylamide) phase transition, creating a hydrogel that blocked a channel or immobilized a single cell. The heaters were activated in sets to redirect flow and exchange the fluid in which an immobilized cell was immersed. A yeast cell was immobilized in hydrogel and a 4',6-diamidino-2-phenylindole (DAPI) fluorescent stain was introduced using flow redirection. DAPI diffused through the hydrogel and fluorescently labelled the yeast DNA, demonstrating in situ single cell biochemistry by means of immobilization and fluid exchange.

Original language	English
Journal	Biomicrofluidics
Volume	6
Issue number	4
Pages (from-to)	041101
ISSN	1932-1058
DOIs	https://doi.org/10.1063/1.4768905
Publication status	Published - 2012
Externally published	Yes

Bibliographical note

Keywords

biochemical application
fluid exchange
in situ single cell biochemistry
Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Fungi [15000] yeast common
4',6-diamidino-2-phenylindole DAPI fluorescent stain
DNA
hydrogel 124586-38-5
thermally responsive fluid
02502, Cytology - General
02504, Cytology - Plant
10060, Biochemistry studies - General
10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
10511, Biophysics - Bioengineering
51522, Plant physiology - Chemical constituents
cell immobilization laboratory techniques
flow manipulation laboratory techniques
flow redirection laboratory techniques
Biochemistry and Molecular Biophysics
Biomaterials
Cell Biology
Methods and Techniques

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BIOMGB 000006 041101 1Final published version, 788 KB

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abstract = "A flow redirection and single cell immobilization method in a microfluidic chip is presented. Microheaters generated localized heating and induced poly(N-isopropylacrylamide) phase transition, creating a hydrogel that blocked a channel or immobilized a single cell. The heaters were activated in sets to redirect flow and exchange the fluid in which an immobilized cell was immersed. A yeast cell was immobilized in hydrogel and a 4',6-diamidino-2-phenylindole (DAPI) fluorescent stain was introduced using flow redirection. DAPI diffused through the hydrogel and fluorescently labelled the yeast DNA, demonstrating in situ single cell biochemistry by means of immobilization and fluid exchange.",

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AU - Thilsted, Anil Haraksingh

AU - Bazargan, Vahid

AU - Piggott, Nina

AU - Measday, Vivien

AU - Stoeber, Boris

PY - 2012

Y1 - 2012

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AB - A flow redirection and single cell immobilization method in a microfluidic chip is presented. Microheaters generated localized heating and induced poly(N-isopropylacrylamide) phase transition, creating a hydrogel that blocked a channel or immobilized a single cell. The heaters were activated in sets to redirect flow and exchange the fluid in which an immobilized cell was immersed. A yeast cell was immobilized in hydrogel and a 4',6-diamidino-2-phenylindole (DAPI) fluorescent stain was introduced using flow redirection. DAPI diffused through the hydrogel and fluorescently labelled the yeast DNA, demonstrating in situ single cell biochemistry by means of immobilization and fluid exchange.

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Flow manipulation and cell immobilization for biochemical applications using thermally responsive fluids

Abstract

Bibliographical note

Keywords

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