Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

  • Author: You, Le-Xing

    Chinese Academy of Sciences, China

  • Author: Liu, Li-Dan

    Chinese Academy of Sciences, China

  • Author: Xiao, Yong

    NanoChemistry, Department of Chemistry, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Dai, You-Fen

    Chinese Academy of Sciences, China

  • Author: Chen, Bi-Lian

    Fujian Normal University, China

  • Author: Jiang, Yan-Xia

    Xiamen University, China

  • Author: Zhao, Feng

    Chinese Academy of Sciences, China

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The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E-t and amperometric I-t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100 nM exogenetic flavins into microbial fuel cells inoculated with LLD-1. Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100 nM exogenetic flavins. This study indicated that flavins were essential to the EET process of the Gram-positive strain LLD-1. Furthermore, a putative EET model for B. megaterium strain LLD-1 and even for Gram-positive bacteria was proposed.
Original languageEnglish
JournalBioelectrochemistry
Volume119
Pages (from-to)196-202
ISSN1567-5394
DOIs
Publication statusPublished - 2018
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Extracellular electron transfer, Microbial fuel cells, Electron shuttle, Flavin, Bacillus megaterium

ID: 138476852