Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1

Le-Xing You, Li-Dan Liu, Yong Xiao*, You-Fen Dai, Bi-Lian Chen, Yan-Xia Jiang, Feng Zhao

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E-t and amperometric I-t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100 nM exogenetic flavins into microbial fuel cells inoculated with LLD-1. Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100 nM exogenetic flavins. This study indicated that flavins were essential to the EET process of the Gram-positive strain LLD-1. Furthermore, a putative EET model for B. megaterium strain LLD-1 and even for Gram-positive bacteria was proposed.
Original languageEnglish
JournalBioelectrochemistry
Volume119
Pages (from-to)196-202
ISSN1567-5394
DOIs
Publication statusPublished - 2018

Keywords

  • Extracellular electron transfer
  • Microbial fuel cells
  • Electron shuttle
  • Flavin
  • Bacillus megaterium

Cite this

You, Le-Xing ; Liu, Li-Dan ; Xiao, Yong ; Dai, You-Fen ; Chen, Bi-Lian ; Jiang, Yan-Xia ; Zhao, Feng. / Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1. In: Bioelectrochemistry. 2018 ; Vol. 119. pp. 196-202.
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title = "Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1",
abstract = "The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E-t and amperometric I-t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100 nM exogenetic flavins into microbial fuel cells inoculated with LLD-1. Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100 nM exogenetic flavins. This study indicated that flavins were essential to the EET process of the Gram-positive strain LLD-1. Furthermore, a putative EET model for B. megaterium strain LLD-1 and even for Gram-positive bacteria was proposed.",
keywords = "Extracellular electron transfer, Microbial fuel cells, Electron shuttle, Flavin, Bacillus megaterium",
author = "Le-Xing You and Li-Dan Liu and Yong Xiao and You-Fen Dai and Bi-Lian Chen and Yan-Xia Jiang and Feng Zhao",
year = "2018",
doi = "10.1016/j.bioelechem.2017.10.005",
language = "English",
volume = "119",
pages = "196--202",
journal = "Bioelectrochemistry",
issn = "1567-5394",
publisher = "Elsevier",

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Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1. / You, Le-Xing; Liu, Li-Dan; Xiao, Yong; Dai, You-Fen; Chen, Bi-Lian; Jiang, Yan-Xia; Zhao, Feng.

In: Bioelectrochemistry, Vol. 119, 2018, p. 196-202.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Flavins mediate extracellular electron transfer in Gram-positive Bacillus megaterium strain LLD-1

AU - You, Le-Xing

AU - Liu, Li-Dan

AU - Xiao, Yong

AU - Dai, You-Fen

AU - Chen, Bi-Lian

AU - Jiang, Yan-Xia

AU - Zhao, Feng

PY - 2018

Y1 - 2018

N2 - The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E-t and amperometric I-t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100 nM exogenetic flavins into microbial fuel cells inoculated with LLD-1. Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100 nM exogenetic flavins. This study indicated that flavins were essential to the EET process of the Gram-positive strain LLD-1. Furthermore, a putative EET model for B. megaterium strain LLD-1 and even for Gram-positive bacteria was proposed.

AB - The extracellular electron transfer (EET) mechanism of an isolated Gram-positive Bacillus megaterium strain (LLD-1), identified by 16S rRNA gene sequencing and physiological analysis, was investigated in the present study. The electrochemical activity of strain LLD-1 was confirmed by electrochemical E-t and amperometric I-t tests. Flavins in culture suspension from strain LLD-1 were further proved to be able to act as electron shuttles, strengthening the electron transfer from LLD-1 to the electrode. The output voltage and current output were increased 2.8 times and 3.7 times, respectively, by adding 100 nM exogenetic flavins into microbial fuel cells inoculated with LLD-1. Electricity generation by LLD-1 from different carbon sources can be enhanced by adding 100 nM exogenetic flavins. This study indicated that flavins were essential to the EET process of the Gram-positive strain LLD-1. Furthermore, a putative EET model for B. megaterium strain LLD-1 and even for Gram-positive bacteria was proposed.

KW - Extracellular electron transfer

KW - Microbial fuel cells

KW - Electron shuttle

KW - Flavin

KW - Bacillus megaterium

U2 - 10.1016/j.bioelechem.2017.10.005

DO - 10.1016/j.bioelechem.2017.10.005

M3 - Journal article

VL - 119

SP - 196

EP - 202

JO - Bioelectrochemistry

JF - Bioelectrochemistry

SN - 1567-5394

ER -