First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe

M Wasniewski, I Almeida, A Baur, T Bedekovic, D Boncea, L B Chaves, D David, P De Benedictis, M Dobrostana, P Giraud, P Hostnik, I Jaceviciene, S Kenklies, M König, K Mähar, M Mojzis, S Moore, S Mrenoski, T Müller, E Ngoepe & 7 others M Nishimura, T Nokireki, N Pejovic, M Smreczak, Bertel Strandbygaard, E Wodak, F Cliquet

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The most effective and sustainable method to control and eliminate rabies in wildlife is the oral rabies vaccination (ORV) of target species, namely foxes and raccoon dogs in Europe. According to WHO and OIE, the effectiveness of oral vaccination campaigns should be regularly assessed via disease surveillance and ORV antibody monitoring. Rabies antibodies are generally screened for in field animal cadavers, whose body fluids are often of poor quality. Therefore, the use of alternative methods such as the enzyme-linked immunosorbent assay (ELISA) has been proposed to improve reliability of serological results obtained on wildlife samples. We undertook an international collaborative study to determine if the commercial BioPro ELISA Rabies Ab kit is a reliable and reproducible tool for rabies serological testing. Our results reveal that the overall specificity evaluated on naive samples reached 96.7%, and the coefficients of concordance obtained for fox and raccoon dog samples were 97.2% and 97.5%, respectively. The overall agreement values obtained for the four marketed oral vaccines used in Europe were all equal to or greater than 95%. The coefficients of concordance obtained by laboratories ranged from 87.2% to 100%. The results of this collaborative study show good robustness and reproducibility of the BioPro ELISA Rabies Ab kit.
    Original languageEnglish
    JournalJournal of Virological Methods
    Volume238
    Pages (from-to)77-85
    ISSN0166-0934
    DOIs
    Publication statusPublished - 2016

    Keywords

    • ELISA
    • collaborative study
    • oral vaccination campaigns
    • rabies
    • rabies antibodies

    Cite this

    Wasniewski, M ; Almeida, I ; Baur, A ; Bedekovic, T ; Boncea, D ; Chaves, L B ; David, D ; De Benedictis, P ; Dobrostana, M ; Giraud, P ; Hostnik, P ; Jaceviciene, I ; Kenklies, S ; König, M ; Mähar, K ; Mojzis, M ; Moore, S ; Mrenoski, S ; Müller, T ; Ngoepe, E ; Nishimura, M ; Nokireki, T ; Pejovic, N ; Smreczak, M ; Strandbygaard, Bertel ; Wodak, E ; Cliquet, F. / First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe. In: Journal of Virological Methods. 2016 ; Vol. 238. pp. 77-85.
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    abstract = "The most effective and sustainable method to control and eliminate rabies in wildlife is the oral rabies vaccination (ORV) of target species, namely foxes and raccoon dogs in Europe. According to WHO and OIE, the effectiveness of oral vaccination campaigns should be regularly assessed via disease surveillance and ORV antibody monitoring. Rabies antibodies are generally screened for in field animal cadavers, whose body fluids are often of poor quality. Therefore, the use of alternative methods such as the enzyme-linked immunosorbent assay (ELISA) has been proposed to improve reliability of serological results obtained on wildlife samples. We undertook an international collaborative study to determine if the commercial BioPro ELISA Rabies Ab kit is a reliable and reproducible tool for rabies serological testing. Our results reveal that the overall specificity evaluated on naive samples reached 96.7{\%}, and the coefficients of concordance obtained for fox and raccoon dog samples were 97.2{\%} and 97.5{\%}, respectively. The overall agreement values obtained for the four marketed oral vaccines used in Europe were all equal to or greater than 95{\%}. The coefficients of concordance obtained by laboratories ranged from 87.2{\%} to 100{\%}. The results of this collaborative study show good robustness and reproducibility of the BioPro ELISA Rabies Ab kit.",
    keywords = "ELISA, collaborative study, oral vaccination campaigns, rabies, rabies antibodies",
    author = "M Wasniewski and I Almeida and A Baur and T Bedekovic and D Boncea and Chaves, {L B} and D David and {De Benedictis}, P and M Dobrostana and P Giraud and P Hostnik and I Jaceviciene and S Kenklies and M K{\"o}nig and K M{\"a}har and M Mojzis and S Moore and S Mrenoski and T M{\"u}ller and E Ngoepe and M Nishimura and T Nokireki and N Pejovic and M Smreczak and Bertel Strandbygaard and E Wodak and F Cliquet",
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    Wasniewski, M, Almeida, I, Baur, A, Bedekovic, T, Boncea, D, Chaves, LB, David, D, De Benedictis, P, Dobrostana, M, Giraud, P, Hostnik, P, Jaceviciene, I, Kenklies, S, König, M, Mähar, K, Mojzis, M, Moore, S, Mrenoski, S, Müller, T, Ngoepe, E, Nishimura, M, Nokireki, T, Pejovic, N, Smreczak, M, Strandbygaard, B, Wodak, E & Cliquet, F 2016, 'First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe', Journal of Virological Methods, vol. 238, pp. 77-85. https://doi.org/10.1016/j.jviromet.2016.10.006

    First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe. / Wasniewski, M; Almeida, I; Baur, A; Bedekovic, T; Boncea, D; Chaves, L B; David, D; De Benedictis, P; Dobrostana, M; Giraud, P; Hostnik, P; Jaceviciene, I; Kenklies, S; König, M; Mähar, K; Mojzis, M; Moore, S; Mrenoski, S; Müller, T; Ngoepe, E; Nishimura, M; Nokireki, T; Pejovic, N; Smreczak, M; Strandbygaard, Bertel; Wodak, E; Cliquet, F.

    In: Journal of Virological Methods, Vol. 238, 2016, p. 77-85.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - First international collaborative study to evaluate rabies antibody detection method for use in monitoring the effectiveness of oral vaccination programmes in fox and raccoon dog in Europe

    AU - Wasniewski, M

    AU - Almeida, I

    AU - Baur, A

    AU - Bedekovic, T

    AU - Boncea, D

    AU - Chaves, L B

    AU - David, D

    AU - De Benedictis, P

    AU - Dobrostana, M

    AU - Giraud, P

    AU - Hostnik, P

    AU - Jaceviciene, I

    AU - Kenklies, S

    AU - König, M

    AU - Mähar, K

    AU - Mojzis, M

    AU - Moore, S

    AU - Mrenoski, S

    AU - Müller, T

    AU - Ngoepe, E

    AU - Nishimura, M

    AU - Nokireki, T

    AU - Pejovic, N

    AU - Smreczak, M

    AU - Strandbygaard, Bertel

    AU - Wodak, E

    AU - Cliquet, F

    PY - 2016

    Y1 - 2016

    N2 - The most effective and sustainable method to control and eliminate rabies in wildlife is the oral rabies vaccination (ORV) of target species, namely foxes and raccoon dogs in Europe. According to WHO and OIE, the effectiveness of oral vaccination campaigns should be regularly assessed via disease surveillance and ORV antibody monitoring. Rabies antibodies are generally screened for in field animal cadavers, whose body fluids are often of poor quality. Therefore, the use of alternative methods such as the enzyme-linked immunosorbent assay (ELISA) has been proposed to improve reliability of serological results obtained on wildlife samples. We undertook an international collaborative study to determine if the commercial BioPro ELISA Rabies Ab kit is a reliable and reproducible tool for rabies serological testing. Our results reveal that the overall specificity evaluated on naive samples reached 96.7%, and the coefficients of concordance obtained for fox and raccoon dog samples were 97.2% and 97.5%, respectively. The overall agreement values obtained for the four marketed oral vaccines used in Europe were all equal to or greater than 95%. The coefficients of concordance obtained by laboratories ranged from 87.2% to 100%. The results of this collaborative study show good robustness and reproducibility of the BioPro ELISA Rabies Ab kit.

    AB - The most effective and sustainable method to control and eliminate rabies in wildlife is the oral rabies vaccination (ORV) of target species, namely foxes and raccoon dogs in Europe. According to WHO and OIE, the effectiveness of oral vaccination campaigns should be regularly assessed via disease surveillance and ORV antibody monitoring. Rabies antibodies are generally screened for in field animal cadavers, whose body fluids are often of poor quality. Therefore, the use of alternative methods such as the enzyme-linked immunosorbent assay (ELISA) has been proposed to improve reliability of serological results obtained on wildlife samples. We undertook an international collaborative study to determine if the commercial BioPro ELISA Rabies Ab kit is a reliable and reproducible tool for rabies serological testing. Our results reveal that the overall specificity evaluated on naive samples reached 96.7%, and the coefficients of concordance obtained for fox and raccoon dog samples were 97.2% and 97.5%, respectively. The overall agreement values obtained for the four marketed oral vaccines used in Europe were all equal to or greater than 95%. The coefficients of concordance obtained by laboratories ranged from 87.2% to 100%. The results of this collaborative study show good robustness and reproducibility of the BioPro ELISA Rabies Ab kit.

    KW - ELISA

    KW - collaborative study

    KW - oral vaccination campaigns

    KW - rabies

    KW - rabies antibodies

    U2 - 10.1016/j.jviromet.2016.10.006

    DO - 10.1016/j.jviromet.2016.10.006

    M3 - Journal article

    VL - 238

    SP - 77

    EP - 85

    JO - Journal of Virological Methods

    JF - Journal of Virological Methods

    SN - 0166-0934

    ER -