Fed-Batch Feeding Strategies for Enzymatic Biodiesel Production

Jason Anthony Price, Mathias Nordblad, John Woodley, Jakob Kjøbsted Huusom

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In this work a kinetic model for the enzymatic transesterification of rapeseed oil using a solubilised lipase (Callera Trans L-Thermomyces lanuginos us) was developed from first principles. The model is based on a Ping-Pong Bi-Bi mechanism, with methanol inhibition, along with consideration of the differences in the interfacial and bulk concentrations of the enzyme. The model is then used to evaluate various feeding strategies to improve the enzymatic biodiesel production. The feeding strategies investigated, gave insight into how the methanol should be fed to potentially mitigate enzyme deactivation while improving the biodiesel yield. The best experimental results gave a yield of 703 .76 g FAME L-1 and a reactor productivity of 28.12 g FAME L-1 h-1. In comparison, to reach the same yield, the optimised two step feeding strategy took 6.25 hours less, which equates to an increase the reactor productivity of 36.9 % .
Original languageEnglish
Title of host publicationPreprints of the 19th World Congress The International Federation of Automatic Control.
Publication date2014
Publication statusPublished - 2014
Event19th World Congress of the International Federation of Automatic Control (IFAC 2014) - Cape Town, South Africa
Duration: 24 Aug 201429 Aug 2014


Conference19th World Congress of the International Federation of Automatic Control (IFAC 2014)
Country/TerritorySouth Africa
CityCape Town
OtherThe theme of the congress: “Promoting automatic control for the benefit of humankind”
Internet address


  • Modelling
  • Sensitivity Analysis


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