Feature-based prediction of non-classical and leaderless protein secretion

Jannick Dyrløv Bendtsen, Lars Juhl Jensen, Nikolaj Blom, G. von Heijne, Søren Brunak

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We present a sequence-based method, SecretomeP, for the prediction of mammalian secretory proteins targeted to the non-classical secretory pathway, i.e. proteins without an N-terminal signal peptide. So far only a limited number of proteins have been shown experimentally to enter the non-classical secretory pathway. These are mainly fibroblast growth factors, interleukins and galectins found in the extracellular matrix. We have discovered that certain pathway-independent features are shared among secreted proteins. The method presented here is also capable of predicting (signal peptide-containing) secretory proteins where only the mature part of the protein has been annotated or cases where the signal peptide remains uncleaved. By scanning the entire human proteome we identified new proteins potentially undergoing non-classical secretion. Predictions can be made at http://www.cbs.dtu.dk/services/SecretomeP.
    Original languageEnglish
    JournalProtein Engineering Design & Selection
    Volume17
    Issue number4
    Pages (from-to)349-356
    Publication statusPublished - 2004

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