Fatty acid biosynthesis in eukaryotic photosynthetic microalgae: Identification of a microsomal delta 12 desaturase in Chlamydomonas reinhardtii

Xiaoyuan Chi, Xiaowen Zhang, Xiangyu Guan, Ling Ding, Youxun Li, Mingqing Wang, Hanzhi Lin, Song Qin

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Polyunsaturated fatty acids (PUFAs) are important components of infant and adult nutrition because they serve as structural elements of cell membranes. Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, the genome-wide characterization of the fatty acid desaturases from seven eukaryotic photosynthetic microalgae was undertaken according to the conserved histidine-rich motifs and phylogenetic profiles. Analysis of these genomes provided insight into the origin and evolution of the pathway of fatty acid biosynthesis in eukaryotic plants. In addition, the candidate enzyme from Chlamydomonas reinhardtii with the highest similarity to the microsomal Delta 12 desaturase of Chlorella vulgaris was isolated, and its function was verified by heterologous expression in yeast (Saccharomyces cerevisiae).
Original languageEnglish
JournalJournal of Basic Microbiology
Volume46
Issue number2
Pages (from-to)189-201
Number of pages13
ISSN0233-111X
DOIs
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • Chlamydomonas reinhardtii
  • Eukaryotic photosynthetic microalgae
  • Fatty acid desaturase

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