Fate and effect of ingested Bacillus cereus spores and vegetative cells in the intestinal tract of human-flora-associated rats

Andrea Wilcks, Bjarne Munk Hansen, Niels Bohse Hendriksen, Tine Rask Licht

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The fate and effect of Bacillus cereus F4433/73R in the intestine of human-flora-associated rats was studied using bacteriological culturing techniques and PCR-denaturing gradient gel electrophoresis in combination with cell assays and immunoassays for detection of enterotoxins. In faecal samples from animals receiving vegetative cells, only few B. cereus cells were detected. Spores survived the gastric barrier well, and were in some cases detected up to 2 weeks after ingestion. Selective growing revealed no major changes in the intestinal flora during passage of B. cereus. However, denaturing gradient gel electrophoresis analysis with universal 16S rRNA gene primers revealed significant changes in the intestinal microbiota of animals dosed with spores. Vero cell assays and a commercial kit (BCET-RPLA) did not reveal any enterotoxin production from B. cereus F4433/73R in the intestinal tract.
Original languageEnglish
JournalFems Immunology and Medical Microbiology
Volume46
Issue number1
Pages (from-to)70-77
ISSN0928-8244
DOIs
Publication statusPublished - 2006

Keywords

  • DGGE
  • spores
  • Bacillus cereus
  • human-flora-associated rats

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