Fast and robust methods for full genome sequencing of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Type 1 and Type 2

Lise Kirstine Kvisgaard, Charlotte Kristiane Hjulsager, Ulrik Fahnøe, Solvej Østergaard Breum, Tahar Ait-Ali, Lars Erik Larsen

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    Abstract

    The high level of diversity among PRRS viruses makes it very important to monitor the overall genetic variations in relation to the sensitivity of diagnostic tests and vaccination efficacy, but only few full genome sequences of PRRSV strains isolated in Europe have been made public available. In the present study, fast and robust methods for long range RT-PCR amplification and subsequent next generation sequencing (NGS) of PRRSV Type 1 and Type 2 viruses were developed and validated on nine Type 1 and nine Type 2 PRRSV viruses. The methods were shown to generate robust and reliable sequences both on primary material and cell culture adapted viruses and the protocols were shown to perform well on all three NGS platforms tested (Roche 454 FLX, Illumina HiSeq 2000, and Ion Torrent PGM™ Sequencer). To complete the sequences at the 5’ end, 5’ Rapid Amplification of cDNA Ends (5’ RACE) was conducted followed by cycle sequencing of clones. The genome lengths were determined to be 14,876-15,098 and 15,342-15,408 nucleotides long for the Type 1 and Type 2 strains, respectively. These methods will greatly facilitate the generation of more complete genome PRRSV sequences globally which in turn may lead to identification of markers of virulence and improve our understanding of PRRSV evolution and pathogenesis.
    Original languageEnglish
    Publication date2013
    Number of pages1
    Publication statusPublished - 2013
    EventInternational Porcine Reproductive and Respiratory Syndrome Symposium (PRRS 2013) - Beijing, China
    Duration: 20 May 201322 May 2013
    http://www.prrssymposium.org/

    Conference

    ConferenceInternational Porcine Reproductive and Respiratory Syndrome Symposium (PRRS 2013)
    CountryChina
    CityBeijing
    Period20/05/201322/05/2013
    Internet address

    Bibliographical note

    Poster presentation.

    Cite this

    Kvisgaard, L. K., Hjulsager, C. K., Fahnøe, U., Breum, S. Ø., Ait-Ali, T., & Larsen, L. E. (2013). Fast and robust methods for full genome sequencing of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Type 1 and Type 2. Abstract from International Porcine Reproductive and Respiratory Syndrome Symposium (PRRS 2013), Beijing, China.