Factor Xa subsite mapping by proteome-derived peptide libraries improved using WebPICS, a resource for proteomic identification of cleavage sites

Oliver Schilling*, Ulrich Auf Dem Keller, Christopher M. Overall

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based data-analysis resource termed WebPics (http://clipserve.clip.ubc.ca/ pics/). We demonstrate the utility of WebPics analysis of PICS data by determining the substrate specificity of factor Xa from P6-P6', an important blood coagulation protease that proteolytically generates thrombin from prothrombin. PICS confirms existing data on non-prime site specificity and refines our knowledge of factor Xa prime-site selectivity.

Original languageEnglish
JournalBiological Chemistry
Volume392
Issue number11
Pages (from-to)1031-1037
ISSN1431-6730
DOIs
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • Peptide library
  • Prime-site specificity
  • Protease specificity

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