Fabrication of phospholipid-xanthan microcapsules by combining microfluidics with self-assembly

Ana Carina Loureiro Mendes, E. T. Baran, R. L. Reis, H. S. Azevedo

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We report the synthesis of an amphiphilic polysaccharide, a phospholipid (1,2-dioleoyl-sn-glycerophosphoetilamine, DOPE) conjugated with the anionic xanthan gum, and its ability to spontaneously self-assemble under mild aqueous conditions. This work also aimed to apply a microfluidic platform that can precisely fabricate microsized and monodispersed capsules for cell encapsulation. Stable hollow capsular structures were obtained by the generation of homogeneous spherical droplets of the self-assembled polymer in the microfluidic device through the formation of a water-in-oil emulsion, followed by the stabilization of the polymer aggregates in a separate collection vessel containing phosphate-buffered saline (physiological ionic strength and pH). The properties (size, morphology, permeability) and performance (stability) of the obtained microcapsules were studied, as well their ability to support the viability, function and proliferation of encapsulated cells. ATDC5 cells were encapsulated within the capsules and shown to remain viable, evidencing increased cellular metabolic activity over 21 days of in vitro culture. By combining microfluidic droplet generation and self-assembly of xanthan-DOPE, we were able to fabricate microcapsules that provided an adequate environment for cells to survive and proliferate. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalActa Biomaterialia
Volume9
Issue number5
Pages (from-to)6675-6685
Number of pages11
ISSN1742-7061
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • ENGINEERING,
  • MATERIALS
  • ALGINATE MICROCAPSULES
  • NANOPARTICLES
  • ENCAPSULATION
  • BIOMATERIALS
  • INDUCTION
  • VESICLES
  • NANOGEL
  • DESIGN
  • MANNAN
  • Self-assembly
  • Hydrophobic
  • Xanthan gum
  • Microfluidics
  • Microcapsule
  • Cell encapsulation
  • 1,2 dioleoyl sn glycerophosphoetilamine
  • phospholipid
  • polymer
  • sodium chloride
  • unclassified drug
  • xanthan
  • article
  • cell encapsulation
  • cell membrane permeability
  • cell metabolism
  • chemical structure
  • conjugation
  • emulsion
  • in vitro study
  • ionic strength
  • microcapsule
  • microfluidics
  • nanofabrication
  • particle size
  • pH
  • priority journal
  • synthesis
  • Cell Line
  • Magnetic Resonance Spectroscopy
  • Microscopy, Electron, Scanning Transmission
  • Phospholipids
  • Polysaccharides, Bacterial
  • 11138-66-2 xanthan gum

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