Fabrication of microstructured surface topologies for the promotion of marine bacteria biofilm

Ariadni Droumpali, Jörg Hübner, Lone Gram, Rafael Taboryski*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Several marine bacteria of the Roseobacter group can inhibit other microorganisms and are especially antagonistic when growing in biofilms. This aptitude to naturally compete with other bacteria can reduce the need for antibiotics in large-scale aquaculture units, provided that their culture can be promoted and controlled. Micropatterned surfaces may facilitate and promote the biofilm formation of species from the Roseobacter group, due to the increased contact between the cells and the surface material. Our research goal is to fabricate biofilm-optimal micropatterned surfaces and investigate the relevant length scales for surface topographies that can promote the growth and biofilm formation of the Roseobacter group of bacteria. In a preliminary study, silicon surfaces comprising arrays of pillars and pits with different periodicities, diameters, and depths were produced by UV lithography and deep reactive ion etching (DRIE) on polished silicon wafers. The resulting surface microscale topologies were characterized via optical profilometry and scanning electron microscopy (SEM). Screening of the bacterial biofilm on the patterned surfaces was performed using green fluorescent staining (SYBR green I) and confocal laser scanning microscopy (CLSM). Our results indicate that there is a correlation between the surface morphology and the spatial organization of the bacterial biofilm.

Original languageEnglish
Article number926
Issue number8
Number of pages10
Publication statusPublished - 2021


  • Bacterial biofilm
  • Microbial adhesion
  • Microfabrication
  • Silicon surfaces
  • Structured surfaces


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