Fabrication of an all-polymer electrochemical sensor by using a one-step hot embossing procedure.

We present a fast one-step hot embossing procedure for fabricating an all-polymer electrochemical sen¬sor based on a thin, conductive film of poly(3,4-ethylenedioxythiophene) (PEDOT), a few 100s of nano¬meters in thickness, polymerised on top of a non-conductive TOPAS® (Cyclic Olefin Copolymer) carrier material. A microfluidic channel was embossed into this two component system. Working (WE) and counter (CE) electrode were integrated into the channel by embossing slopes into the channel sidewalls. Since both electrodes were placed at opposite channel sidewalls, electrical disconnection of electrodes was achieved by spatial separation (Fig. 1). Functionality of the electrochemical system was shown by amperometric detection of physiologically relevant glucose concentrations (0–10 mM).