Fabrication, characterization, and biocompatibility assessment of a novel elastomeric nanofibrous scaffold: A potential scaffold for soft tissue engineering

Elham Shamirzaei Jeshvaghani, Laleh Ghasemi-Mobarakeh*, Reza Mansurnezhad, Fatemeh Ajalloueian, Mahshid Kharaziha, Mohammad Dinari, Maryam Sami Jokandan, Ioannis S. Chronakis

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

With regard to flexibility and strength properties requirements of soft biological tissue, elastomeric materials could be more beneficial in soft tissue engineering applications. The present work investigates the use of an elastic polymer, (polycaprolactone fumarate [PCLF]), for fabricating an electrospun scaffold. PCLF with number-average molecular weight of 13,284 g/mol was synthetized, electrospun PCLF:polycaprolactone (PCL) (70:30) nanofibrous scaffolds were fabricated and a novel strategy (in situ photo-crosslinking along with wet electrospinning) was applied for crosslinking of PCLF in the structure of PCLF:PCL nanofibers was presented. Sol fraction results, Fourier-transform infrared spectroscopy, and mechanical tests confirmed occurrence of crosslinking reaction. Strain at break and Young's modulus of crosslinked PCLF:PCL nanofibers fabricated was found to be 114.5 ± 3.9% and 0.6 ± 0.1 MPa, respectively, and dynamic mechanical analysis results revealed elasticity of nanofibers. MTS assay showed biocompatibility of PCLF:PCL (70:30) nanofibrous scaffolds. Our overall results showed that electrospun PCLF:PCL nanofibrous scaffold could be considered as a candidate for further in vitro and in vivo experiments and its application for engineering of soft tissues subjected to in vivo cyclic mechanical stresses.
Original languageEnglish
JournalJournal of Biomedical Materials Research. Part B: Applied Biomaterials
Volume106
Issue number6
Pages (from-to)2371-2383
Number of pages13
ISSN1552-4973
DOIs
Publication statusPublished - 2018

Keywords

  • Elastomer
  • Electrospinning
  • Polycaprolactone
  • Polycaprolactone fumarate
  • Soft tissue engineering

Cite this

Shamirzaei Jeshvaghani, Elham ; Ghasemi-Mobarakeh, Laleh ; Mansurnezhad, Reza ; Ajalloueian, Fatemeh ; Kharaziha, Mahshid ; Dinari, Mohammad ; Sami Jokandan, Maryam ; Chronakis, Ioannis S. / Fabrication, characterization, and biocompatibility assessment of a novel elastomeric nanofibrous scaffold: A potential scaffold for soft tissue engineering. In: Journal of Biomedical Materials Research. Part B: Applied Biomaterials. 2018 ; Vol. 106, No. 6. pp. 2371-2383.
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abstract = "With regard to flexibility and strength properties requirements of soft biological tissue, elastomeric materials could be more beneficial in soft tissue engineering applications. The present work investigates the use of an elastic polymer, (polycaprolactone fumarate [PCLF]), for fabricating an electrospun scaffold. PCLF with number-average molecular weight of 13,284 g/mol was synthetized, electrospun PCLF:polycaprolactone (PCL) (70:30) nanofibrous scaffolds were fabricated and a novel strategy (in situ photo-crosslinking along with wet electrospinning) was applied for crosslinking of PCLF in the structure of PCLF:PCL nanofibers was presented. Sol fraction results, Fourier-transform infrared spectroscopy, and mechanical tests confirmed occurrence of crosslinking reaction. Strain at break and Young's modulus of crosslinked PCLF:PCL nanofibers fabricated was found to be 114.5 ± 3.9{\%} and 0.6 ± 0.1 MPa, respectively, and dynamic mechanical analysis results revealed elasticity of nanofibers. MTS assay showed biocompatibility of PCLF:PCL (70:30) nanofibrous scaffolds. Our overall results showed that electrospun PCLF:PCL nanofibrous scaffold could be considered as a candidate for further in vitro and in vivo experiments and its application for engineering of soft tissues subjected to in vivo cyclic mechanical stresses.",
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author = "{Shamirzaei Jeshvaghani}, Elham and Laleh Ghasemi-Mobarakeh and Reza Mansurnezhad and Fatemeh Ajalloueian and Mahshid Kharaziha and Mohammad Dinari and {Sami Jokandan}, Maryam and Chronakis, {Ioannis S.}",
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Fabrication, characterization, and biocompatibility assessment of a novel elastomeric nanofibrous scaffold: A potential scaffold for soft tissue engineering. / Shamirzaei Jeshvaghani, Elham; Ghasemi-Mobarakeh, Laleh; Mansurnezhad, Reza; Ajalloueian, Fatemeh; Kharaziha, Mahshid; Dinari, Mohammad; Sami Jokandan, Maryam; Chronakis, Ioannis S.

In: Journal of Biomedical Materials Research. Part B: Applied Biomaterials, Vol. 106, No. 6, 2018, p. 2371-2383.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Fabrication, characterization, and biocompatibility assessment of a novel elastomeric nanofibrous scaffold: A potential scaffold for soft tissue engineering

AU - Shamirzaei Jeshvaghani, Elham

AU - Ghasemi-Mobarakeh, Laleh

AU - Mansurnezhad, Reza

AU - Ajalloueian, Fatemeh

AU - Kharaziha, Mahshid

AU - Dinari, Mohammad

AU - Sami Jokandan, Maryam

AU - Chronakis, Ioannis S.

PY - 2018

Y1 - 2018

N2 - With regard to flexibility and strength properties requirements of soft biological tissue, elastomeric materials could be more beneficial in soft tissue engineering applications. The present work investigates the use of an elastic polymer, (polycaprolactone fumarate [PCLF]), for fabricating an electrospun scaffold. PCLF with number-average molecular weight of 13,284 g/mol was synthetized, electrospun PCLF:polycaprolactone (PCL) (70:30) nanofibrous scaffolds were fabricated and a novel strategy (in situ photo-crosslinking along with wet electrospinning) was applied for crosslinking of PCLF in the structure of PCLF:PCL nanofibers was presented. Sol fraction results, Fourier-transform infrared spectroscopy, and mechanical tests confirmed occurrence of crosslinking reaction. Strain at break and Young's modulus of crosslinked PCLF:PCL nanofibers fabricated was found to be 114.5 ± 3.9% and 0.6 ± 0.1 MPa, respectively, and dynamic mechanical analysis results revealed elasticity of nanofibers. MTS assay showed biocompatibility of PCLF:PCL (70:30) nanofibrous scaffolds. Our overall results showed that electrospun PCLF:PCL nanofibrous scaffold could be considered as a candidate for further in vitro and in vivo experiments and its application for engineering of soft tissues subjected to in vivo cyclic mechanical stresses.

AB - With regard to flexibility and strength properties requirements of soft biological tissue, elastomeric materials could be more beneficial in soft tissue engineering applications. The present work investigates the use of an elastic polymer, (polycaprolactone fumarate [PCLF]), for fabricating an electrospun scaffold. PCLF with number-average molecular weight of 13,284 g/mol was synthetized, electrospun PCLF:polycaprolactone (PCL) (70:30) nanofibrous scaffolds were fabricated and a novel strategy (in situ photo-crosslinking along with wet electrospinning) was applied for crosslinking of PCLF in the structure of PCLF:PCL nanofibers was presented. Sol fraction results, Fourier-transform infrared spectroscopy, and mechanical tests confirmed occurrence of crosslinking reaction. Strain at break and Young's modulus of crosslinked PCLF:PCL nanofibers fabricated was found to be 114.5 ± 3.9% and 0.6 ± 0.1 MPa, respectively, and dynamic mechanical analysis results revealed elasticity of nanofibers. MTS assay showed biocompatibility of PCLF:PCL (70:30) nanofibrous scaffolds. Our overall results showed that electrospun PCLF:PCL nanofibrous scaffold could be considered as a candidate for further in vitro and in vivo experiments and its application for engineering of soft tissues subjected to in vivo cyclic mechanical stresses.

KW - Elastomer

KW - Electrospinning

KW - Polycaprolactone

KW - Polycaprolactone fumarate

KW - Soft tissue engineering

U2 - 10.1002/jbm.b.34043

DO - 10.1002/jbm.b.34043

M3 - Journal article

VL - 106

SP - 2371

EP - 2383

JO - Journal of Biomedical Materials Research. Part B: Applied Biomaterials

JF - Journal of Biomedical Materials Research. Part B: Applied Biomaterials

SN - 1552-4973

IS - 6

ER -