Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane. A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4–1.2 mol/l), alkali treatment time (X2: 6–30 h), enzyme concentration (X3: 15–75 U/mg) and hydrolysis time (X4: 12–60 h) for maximum PSC extraction. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods. According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h. The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.© 2015 Elsevier Ltd. All rights reserved.
- Eggshell membrane
- Response surface methodology
- Pepsin-soluble collagen
Mohammadi, R., Mohammadifar, M. A., Mortazavian, A. M., Rouhi, M., Ghasemi, J. B., & Delshadian, Z. (2016). Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM). Food Chemistry, 190, 186–193. https://doi.org/10.1016/j.foodchem.2015.05.073