TY - JOUR
T1 - Extraction optimization of pepsin-soluble collagen from eggshell membrane by response surface methodology (RSM)
AU - Mohammadi, Reza
AU - Mohammadifar, Mohammad Amin
AU - Mortazavian, Amir Mohammad
AU - Rouhi, Milad
AU - Ghasemi, Jahan B.
AU - Delshadian, Zohre
PY - 2016
Y1 - 2016
N2 - Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane. A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4–1.2 mol/l), alkali treatment time (X2: 6–30 h), enzyme concentration (X3: 15–75 U/mg) and hydrolysis time (X4: 12–60 h) for maximum PSC extraction. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods. According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h. The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.© 2015 Elsevier Ltd. All rights reserved.
AB - Response surface methodology (RSM) was used to investigate the effect of extraction-process variables on pepsin-soluble collagen (PSC) from eggshell membrane. A central composite design (CCD) was employed for experimental design and analysis of the results to obtain the best possible combination of NaOH concentration (X1: 0.4–1.2 mol/l), alkali treatment time (X2: 6–30 h), enzyme concentration (X3: 15–75 U/mg) and hydrolysis time (X4: 12–60 h) for maximum PSC extraction. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and analyzed by appropriate statistical methods. According to the results, optimum extraction conditions were as follows: NaOH concentration of 0.76 mol/l, alkali treatment time of 18 h, enzyme concentration of 50 U/mg and hydrolysis time of 43.42 h. The experimental extraction yield under optimal conditions was found to be 30.049%, which is in good agreement with the predicted value of 30.054%.© 2015 Elsevier Ltd. All rights reserved.
KW - Eggshell membrane
KW - Response surface methodology
KW - Pepsin-soluble collagen
U2 - 10.1016/j.foodchem.2015.05.073
DO - 10.1016/j.foodchem.2015.05.073
M3 - Journal article
C2 - 26212959
SN - 0308-8146
VL - 190
SP - 186
EP - 193
JO - Food Chemistry
JF - Food Chemistry
ER -