Extraction of bioactive compounds froms Lion's Mane mushroom by-product using supercritical CO2 extraction

Pinida Joradon, Vilai Rungsardthong*, Uracha Ruktanonchai, Khomson Suttisintong, Benjawan Thumthanaruk, Savitri Vatanyoopaisarn, Dudsadee Uttapap, Ana C. Mendes

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Supercritical fluid extraction using CO2 (SCFE-CO2) was used to examine extraction efficiency of bioactive compounds from irregular-shaped Lion's Mane (LM) mushroom by-products. Optimal SCFE-CO2 conditions (46.38 °C, 100 bar, and 0.99 mL/min of EtOH flow rate) for maximum yield (a mass fraction of 11.64%), ergosterol (3.74 mg/g dried LM), total phenolic content, TPC (815.93 µg GAE/g dried LM), and antioxidant activity (42.97 µg TE/g dried LM) were determined using following parameters: temperature (40 °C to 60 °C), pressure (100 bar to 300 bar), and EtOH flow rate (0 mL/min to 1 mL/min). EtOH flow rate was a key factor in raising extraction yield and quality. SCFE-CO2 was equally effective compared to conventional extraction (Maceration) for LM, as there were no significant differences (p ≥ 0.05) in yield, ergosterol content, TPC, or antioxidant activity. This green extraction technology could be an excellent alternative method for recovering bioactive compounds from medicinal mushrooms.
Original languageEnglish
Article number106162
JournalJournal of Supercritical Fluids
Number of pages13
Publication statusPublished - 2024


  • Lion’s Mane mushroom
  • Supercritical fluid extraction
  • Conventional extractions
  • Ergosterol
  • Antioxidant activity


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