Abstract
| Original language | English |
|---|---|
| Journal | ACS Sensors |
| Volume | 4 |
| Issue number | 2 |
| Pages (from-to) | 398-405 |
| ISSN | 2379-3694 |
| DOIs | |
| Publication status | Published - 2019 |
Keywords
- Lab-on-a-disc
- Supported liquid membrane extraction
- Electrochemical sensor
- Bacterial metabolite
- Bioprocess monitoring
Cite this
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Extraction, enrichment, and in situ electrochemical detection on lab- on-a-disc; monitoring the production of a bacterial secondary metabolite. / Andreasen, Sune Zoëga; Sanger, Kuldeep; Jendresen, Christian Bille; Nielsen, Alex Toftgaard; Emnéus, Jenny; Boisen, Anja; Zor, Kinga.
In: ACS Sensors, Vol. 4, No. 2, 2019, p. 398-405.Research output: Contribution to journal › Journal article › Research › peer-review
TY - JOUR
T1 - Extraction, enrichment, and in situ electrochemical detection on lab- on-a-disc; monitoring the production of a bacterial secondary metabolite
AU - Andreasen, Sune Zoëga
AU - Sanger, Kuldeep
AU - Jendresen, Christian Bille
AU - Nielsen, Alex Toftgaard
AU - Emnéus, Jenny
AU - Boisen, Anja
AU - Zor, Kinga
PY - 2019
Y1 - 2019
N2 - Development of microsystems, which enable 'sample-to-answer' detection from real samples, is often challenging. We present the first integration of supported liquid membrane extraction combined with electrochemical detection on a centrifugal fluidic platform. The developed lab-on-a-disc (LoD) system enabled the separation, enrichment and subse-quent electrochemical detection of the target analyte from a complex sample mixture. As a case study, we quantified the amount of a dietary supplement and pharmaceutical precursor, p-Coumaric acid, from bacterial growth media at different time points during production. The assay, extraction, and detection, performed on the LoD device, proved to be a low cost and environmentally friendly approach, requiring only a few tens of µL organic solvent and enabled detection in 3 µL volume. In addition, the data obtained from the centrifugal platform showed a good correlation with data obtained from high performance liquid chromatography analysis.
AB - Development of microsystems, which enable 'sample-to-answer' detection from real samples, is often challenging. We present the first integration of supported liquid membrane extraction combined with electrochemical detection on a centrifugal fluidic platform. The developed lab-on-a-disc (LoD) system enabled the separation, enrichment and subse-quent electrochemical detection of the target analyte from a complex sample mixture. As a case study, we quantified the amount of a dietary supplement and pharmaceutical precursor, p-Coumaric acid, from bacterial growth media at different time points during production. The assay, extraction, and detection, performed on the LoD device, proved to be a low cost and environmentally friendly approach, requiring only a few tens of µL organic solvent and enabled detection in 3 µL volume. In addition, the data obtained from the centrifugal platform showed a good correlation with data obtained from high performance liquid chromatography analysis.
KW - Lab-on-a-disc
KW - Supported liquid membrane extraction
KW - Electrochemical sensor
KW - Bacterial metabolite
KW - Bioprocess monitoring
U2 - 10.1021/acssensors.8b01277
DO - 10.1021/acssensors.8b01277
M3 - Journal article
VL - 4
SP - 398
EP - 405
JO - ACS Sensors
JF - ACS Sensors
SN - 2379-3694
IS - 2
ER -