Extraction, enrichment, and in situ electrochemical detection on lab- on-a-disc; monitoring the production of a bacterial secondary metabolite

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Development of microsystems, which enable 'sample-to-answer' detection from real samples, is often challenging. We present the first integration of supported liquid membrane extraction combined with electrochemical detection on a centrifugal fluidic platform. The developed lab-on-a-disc (LoD) system enabled the separation, enrichment and subse-quent electrochemical detection of the target analyte from a complex sample mixture. As a case study, we quantified the amount of a dietary supplement and pharmaceutical precursor, p-Coumaric acid, from bacterial growth media at different time points during production. The assay, extraction, and detection, performed on the LoD device, proved to be a low cost and environmentally friendly approach, requiring only a few tens of µL organic solvent and enabled detection in 3 µL volume. In addition, the data obtained from the centrifugal platform showed a good correlation with data obtained from high performance liquid chromatography analysis.
Original languageEnglish
JournalACS Sensors
Volume4
Issue number2
Pages (from-to)398-405
ISSN2379-3694
DOIs
Publication statusPublished - 2019

Keywords

  • Lab-on-a-disc
  • Supported liquid membrane extraction
  • Electrochemical sensor
  • Bacterial metabolite
  • Bioprocess monitoring

Cite this

@article{a298407bd6454df39be8dd96c86cb05c,
title = "Extraction, enrichment, and in situ electrochemical detection on lab- on-a-disc; monitoring the production of a bacterial secondary metabolite",
abstract = "Development of microsystems, which enable 'sample-to-answer' detection from real samples, is often challenging. We present the first integration of supported liquid membrane extraction combined with electrochemical detection on a centrifugal fluidic platform. The developed lab-on-a-disc (LoD) system enabled the separation, enrichment and subse-quent electrochemical detection of the target analyte from a complex sample mixture. As a case study, we quantified the amount of a dietary supplement and pharmaceutical precursor, p-Coumaric acid, from bacterial growth media at different time points during production. The assay, extraction, and detection, performed on the LoD device, proved to be a low cost and environmentally friendly approach, requiring only a few tens of µL organic solvent and enabled detection in 3 µL volume. In addition, the data obtained from the centrifugal platform showed a good correlation with data obtained from high performance liquid chromatography analysis.",
keywords = "Lab-on-a-disc, Supported liquid membrane extraction, Electrochemical sensor, Bacterial metabolite, Bioprocess monitoring",
author = "Andreasen, {Sune Zo{\"e}ga} and Kuldeep Sanger and Jendresen, {Christian Bille} and Nielsen, {Alex Toftgaard} and Jenny Emn{\'e}us and Anja Boisen and Kinga Zor",
year = "2019",
doi = "10.1021/acssensors.8b01277",
language = "English",
volume = "4",
pages = "398--405",
journal = "ACS Sensors",
issn = "2379-3694",
publisher = "American Chemical Society",
number = "2",

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TY - JOUR

T1 - Extraction, enrichment, and in situ electrochemical detection on lab- on-a-disc; monitoring the production of a bacterial secondary metabolite

AU - Andreasen, Sune Zoëga

AU - Sanger, Kuldeep

AU - Jendresen, Christian Bille

AU - Nielsen, Alex Toftgaard

AU - Emnéus, Jenny

AU - Boisen, Anja

AU - Zor, Kinga

PY - 2019

Y1 - 2019

N2 - Development of microsystems, which enable 'sample-to-answer' detection from real samples, is often challenging. We present the first integration of supported liquid membrane extraction combined with electrochemical detection on a centrifugal fluidic platform. The developed lab-on-a-disc (LoD) system enabled the separation, enrichment and subse-quent electrochemical detection of the target analyte from a complex sample mixture. As a case study, we quantified the amount of a dietary supplement and pharmaceutical precursor, p-Coumaric acid, from bacterial growth media at different time points during production. The assay, extraction, and detection, performed on the LoD device, proved to be a low cost and environmentally friendly approach, requiring only a few tens of µL organic solvent and enabled detection in 3 µL volume. In addition, the data obtained from the centrifugal platform showed a good correlation with data obtained from high performance liquid chromatography analysis.

AB - Development of microsystems, which enable 'sample-to-answer' detection from real samples, is often challenging. We present the first integration of supported liquid membrane extraction combined with electrochemical detection on a centrifugal fluidic platform. The developed lab-on-a-disc (LoD) system enabled the separation, enrichment and subse-quent electrochemical detection of the target analyte from a complex sample mixture. As a case study, we quantified the amount of a dietary supplement and pharmaceutical precursor, p-Coumaric acid, from bacterial growth media at different time points during production. The assay, extraction, and detection, performed on the LoD device, proved to be a low cost and environmentally friendly approach, requiring only a few tens of µL organic solvent and enabled detection in 3 µL volume. In addition, the data obtained from the centrifugal platform showed a good correlation with data obtained from high performance liquid chromatography analysis.

KW - Lab-on-a-disc

KW - Supported liquid membrane extraction

KW - Electrochemical sensor

KW - Bacterial metabolite

KW - Bioprocess monitoring

U2 - 10.1021/acssensors.8b01277

DO - 10.1021/acssensors.8b01277

M3 - Journal article

VL - 4

SP - 398

EP - 405

JO - ACS Sensors

JF - ACS Sensors

SN - 2379-3694

IS - 2

ER -