TY - JOUR
T1 - Extraction and Oxford Nanopore sequencing of genomic DNA from filamentous Actinobacteria
AU - Alvarez-Arevalo, Maria
AU - Sterndorff, Eva Baggesgaard
AU - Faurdal, David
AU - Jørgensen, Tue Sparholt
AU - Mourched, Anna-Sophie
AU - Vuksanovic, Oliwia
AU - Saha, Subhasish
AU - Weber, Tilmann
PY - 2023
Y1 - 2023
N2 - Actinomycetota (Actinobacteria) is an ecologically and industrially important phylum which is challenging to extract pure high-molecular-weight (HMW) DNA from. This protocol provides a parallelized, cost-effective, and straightforward approach for consistently extracting pure HMW DNA using modified non-toxic commercial kits suitable for higher throughput applications. We further provide a workflow for sequencing and assembly of complete genomes using an optimized Oxford Nanopore rapid barcoding protocol and Illumina data error correction.
AB - Actinomycetota (Actinobacteria) is an ecologically and industrially important phylum which is challenging to extract pure high-molecular-weight (HMW) DNA from. This protocol provides a parallelized, cost-effective, and straightforward approach for consistently extracting pure HMW DNA using modified non-toxic commercial kits suitable for higher throughput applications. We further provide a workflow for sequencing and assembly of complete genomes using an optimized Oxford Nanopore rapid barcoding protocol and Illumina data error correction.
U2 - 10.1016/j.xpro.2022.101955
DO - 10.1016/j.xpro.2022.101955
M3 - Journal article
C2 - 36527715
SN - 2666-1667
VL - 4
JO - STAR Protocols
JF - STAR Protocols
IS - 1
M1 - 101955
ER -