Abstract
The aquatic ecosystem represents a large number
of organisms adapted to living conditions
remarkably different from the land-living ones.
For instance fish generally possess an eminent
bioactive defense to protect them from the high
bacterial load in water, and must be expected to
harbor a large number of bio-components such
as bioactive peptides for this purpose.
Tissue and proteins from e.g. fish gills, skin and
viscera could be a new source of peptides that
could have a nutritional and pharmaceutical
value, and be used in health and functional foods
and thereby increasing the value adding of
secondary marine products.
Only few naturally occurring bioactive peptides
have been characterized such as the
antimicrobial polypeptide piscidines from gills.
It is therefore hypothesized, that fish tissue also
contains numerous other peptides with other
bioactive properties.
The approach in this project is therefore to
extract and identify naturally occurring bioactive
peptides from different tissues from salmon.
A number of aqueous extracts were made from
gills, skin and belly flap. In order to preserve the
bioactivity of the peptides mild extraction
procedures as acidic, basic and aqueous
solutions were used. Combination of different
extraction conditions such as with/without
boiling, with/without inhibitor and variation of
pH resulted in a total of 36 extracts. The activity
of the extracts was analyzed in vitro for ACE
(angiotensin-converting enzyme) inhibiting
activity, and anti-oxidative activity (Free Radical
Scavenging assay).
A number of extracts showed high ACE
inhibiting and anti-oxidative activity. The
extracts were then size fractionated by
ultrafiltration using a 10 kDa filter, and relevant
fractions below 10 kDa from gills, skin and belly
flap were further fractionated by gel-filtration on
a Superdex peptide HR 10/30 column. Peptide
fractions were collected and freeze-dried and
will be further characterized with LCMS and
MS/MS.
Original language | English |
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Title of host publication | WEFTA 2011 - Seafood for the Modern Consumer : Abstracts |
Number of pages | 119 |
Place of Publication | Gothenburg |
Publisher | Chalmers tekniska högskola |
Publication date | 2011 |
Publication status | Published - 2011 |
Event | 41st Annual WEFTA Meeting: Seafood for the Modern Consumer - Gothenburg, Sweden Duration: 27 Sept 2011 → 30 Sept 2011 Conference number: 41 http://www.wefta2011.org/ |
Conference
Conference | 41st Annual WEFTA Meeting |
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Number | 41 |
Country/Territory | Sweden |
City | Gothenburg |
Period | 27/09/2011 → 30/09/2011 |
Internet address |