Extraction and characterization of naturally occurring bioactive peptides from different tissues from Salmon (Salmo salar)

Susan Skanderup Falkenberg, Henrik Hauch Nielsen

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearch

Abstract

The aquatic ecosystem represents a large number of organisms adapted to living conditions remarkably different from the land-living ones. For instance fish generally possess an eminent bioactive defense to protect them from the high bacterial load in water, and must be expected to harbor a large number of bio-components such as bioactive peptides for this purpose. Tissue and proteins from e.g. fish gills, skin and viscera could be a new source of peptides that could have a nutritional and pharmaceutical value, and be used in health and functional foods and thereby increasing the value adding of secondary marine products. Only few naturally occurring bioactive peptides have been characterized such as the antimicrobial polypeptide piscidines from gills. It is therefore hypothesized, that fish tissue also contains numerous other peptides with other bioactive properties. The approach in this project is therefore to extract and identify naturally occurring bioactive peptides from different tissues from salmon. A number of aqueous extracts were made from gills, skin and belly flap. In order to preserve the bioactivity of the peptides mild extraction procedures as acidic, basic and aqueous solutions were used. Combination of different extraction conditions such as with/without boiling, with/without inhibitor and variation of pH resulted in a total of 36 extracts. The activity of the extracts was analyzed in vitro for ACE (angiotensin-converting enzyme) inhibiting activity, and anti-oxidative activity (Free Radical Scavenging assay). A number of extracts showed high ACE inhibiting and anti-oxidative activity. The extracts were then size fractionated by ultrafiltration using a 10 kDa filter, and relevant fractions below 10 kDa from gills, skin and belly flap were further fractionated by gel-filtration on a Superdex peptide HR 10/30 column. Peptide fractions were collected and freeze-dried and will be further characterized with LCMS and MS/MS.
Original languageEnglish
Title of host publicationWEFTA 2011 - Seafood for the Modern Consumer : Abstracts
Number of pages119
Place of PublicationGothenburg
PublisherChalmers tekniska högskola
Publication date2011
Publication statusPublished - 2011
Event41st Annual WEFTA Meeting: Seafood for the Modern Consumer - Gothenburg, Sweden
Duration: 27 Sep 201130 Sep 2011
Conference number: 41
http://www.wefta2011.org/

Conference

Conference41st Annual WEFTA Meeting
Number41
CountrySweden
CityGothenburg
Period27/09/201130/09/2011
Internet address

Cite this

Falkenberg, S. S., & Nielsen, H. H. (2011). Extraction and characterization of naturally occurring bioactive peptides from different tissues from Salmon (Salmo salar). In WEFTA 2011 - Seafood for the Modern Consumer: Abstracts Gothenburg: Chalmers tekniska högskola.
Falkenberg, Susan Skanderup ; Nielsen, Henrik Hauch. / Extraction and characterization of naturally occurring bioactive peptides from different tissues from Salmon (Salmo salar). WEFTA 2011 - Seafood for the Modern Consumer: Abstracts. Gothenburg : Chalmers tekniska högskola, 2011.
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Falkenberg, SS & Nielsen, HH 2011, Extraction and characterization of naturally occurring bioactive peptides from different tissues from Salmon (Salmo salar). in WEFTA 2011 - Seafood for the Modern Consumer: Abstracts. Chalmers tekniska högskola, Gothenburg, 41st Annual WEFTA Meeting, Gothenburg, Sweden, 27/09/2011.

Extraction and characterization of naturally occurring bioactive peptides from different tissues from Salmon (Salmo salar). / Falkenberg, Susan Skanderup; Nielsen, Henrik Hauch.

WEFTA 2011 - Seafood for the Modern Consumer: Abstracts. Gothenburg : Chalmers tekniska högskola, 2011.

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsResearch

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AB - The aquatic ecosystem represents a large number of organisms adapted to living conditions remarkably different from the land-living ones. For instance fish generally possess an eminent bioactive defense to protect them from the high bacterial load in water, and must be expected to harbor a large number of bio-components such as bioactive peptides for this purpose. Tissue and proteins from e.g. fish gills, skin and viscera could be a new source of peptides that could have a nutritional and pharmaceutical value, and be used in health and functional foods and thereby increasing the value adding of secondary marine products. Only few naturally occurring bioactive peptides have been characterized such as the antimicrobial polypeptide piscidines from gills. It is therefore hypothesized, that fish tissue also contains numerous other peptides with other bioactive properties. The approach in this project is therefore to extract and identify naturally occurring bioactive peptides from different tissues from salmon. A number of aqueous extracts were made from gills, skin and belly flap. In order to preserve the bioactivity of the peptides mild extraction procedures as acidic, basic and aqueous solutions were used. Combination of different extraction conditions such as with/without boiling, with/without inhibitor and variation of pH resulted in a total of 36 extracts. The activity of the extracts was analyzed in vitro for ACE (angiotensin-converting enzyme) inhibiting activity, and anti-oxidative activity (Free Radical Scavenging assay). A number of extracts showed high ACE inhibiting and anti-oxidative activity. The extracts were then size fractionated by ultrafiltration using a 10 kDa filter, and relevant fractions below 10 kDa from gills, skin and belly flap were further fractionated by gel-filtration on a Superdex peptide HR 10/30 column. Peptide fractions were collected and freeze-dried and will be further characterized with LCMS and MS/MS.

M3 - Conference abstract in proceedings

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PB - Chalmers tekniska högskola

CY - Gothenburg

ER -

Falkenberg SS, Nielsen HH. Extraction and characterization of naturally occurring bioactive peptides from different tissues from Salmon (Salmo salar). In WEFTA 2011 - Seafood for the Modern Consumer: Abstracts. Gothenburg: Chalmers tekniska högskola. 2011