Extracting information from two-dimensional electrophoresis gels by partial least squares regression

Flemming Jessen, R. Lametsch, E. Bendixen, Inger Vibeke Holst Kjærsgård, Bo Jørgensen

Research output: Contribution to journalJournal articleResearchpeer-review


Two-dimensional gel electrophoresis (2-DE) produces large amounts of data and extraction of relevant information from these data demands a cautious and time consuming process of spot pattern matching between gels. The classical approach of data analysis is to detect protein markers that appear or disappear depending on the experimental conditions. Such biomarkers are found by comparing the relative volumes of individual spots in the individual gels. Multivariate statistical analysis and modelling of 2-DE data for comparison and classification is an alternative approach utilising the combination of all proteins/spots in the gels. In the present study it is demonstrated how information can be extracted by multivariate data analysis. The strategy is based on partial least squares regression followed by variable selection to find proteins that individually or in combination with other proteins vary informatively in relation to the experimental conditions. Finding of such coherent protein patterns leads to identification of potential relations between the involved proteins, and will be useful for focusing further investigation of proteins that relate to the chosen experimental conditions.
Original languageEnglish
Issue number1
Pages (from-to)32-35
Publication statusPublished - 2002


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